Assay Detail
Functional
CHEMBL3436205
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of Cav1.2 current measured using QPatch automatic path clamp system in CHO cells expressing Cav1.2, beta-2 and alpha-2/delta-1 subunits
1
Total Activities
1
Compounds Tested
1
Activity Types
6
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Cricetulus griseus |
| Cell Type | CHO |
| Confidence | 4 — Cell-line / Tissue |
| Curated By | Autocuration |
Target
Voltage-gated L-type calcium channel (CHEMBL2095229) ↗| Type | PROTEIN FAMILY |
| Organism | Homo sapiens |
Publication
MICE models: superior to the HERG model in predicting Torsade de Pointes.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 1 | 4.05 | 4.05 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| CA_SOL_CONC | CA_SOL_CONC | 1.8 | mM | — |
| DETECTION_METHOD | DETECTION_METHOD | - | — | QPatch automated patch clamp |
| HOLDING_POTENTIAL | HOLDING_POTENTIAL | - | — | -40 |
| MEAS_VOLT | MEAS_VOLT | - | mV | — |
| T1_PULSE | T1_PULSE | 150.0 | ms | — |
| TEMP | TEMP | - | — | Ambient |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL850 | SPARFLOXACIN | 4.0 | 1 | 4.05 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL850 | SPARFLOXACIN | IC50 | = | 88800.0 | nM | 4.05 |