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Assay Detail

CHEMBL3579074

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity against human recombinant TRPM8 expressed in CHO T-rex cells assessed as inhibition of WS12-induced calcium response pre-incubated for 20 mins before WS12 addition
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO T-rex
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery of a Selective TRPM8 Antagonist with Clinical Efficacy in Cold-Related Pain.
Andrews MD, Af Forselles K, Beaumont K, Galan SR, Glossop PA, Grenie M, Jessiman A, Kenyon AS, Lunn G, Maw G, Owen RM, Pryde DC, Roberts D, Tran TD.
ACS Med Chem Lett (2015) 6 : 419 -424
PubMed 25893043 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 6.43 7.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3577904 1 7.22
CHEMBL3577902 1 7.21
CHEMBL3577889 1 7.05
CHEMBL3577881 1 7.03
CHEMBL3577905 1 6.91
CHEMBL3577883 1 6.84
CHEMBL3577885 PF-05105679 1.0 1 6.74
CHEMBL3577907 1 6.72
CHEMBL3577900 1 6.70
CHEMBL3577906 1 6.63
CHEMBL3577898 1 6.54
CHEMBL3576881 1 6.51
CHEMBL3577903 1 6.48
CHEMBL3577886 1 6.47
CHEMBL3577893 1 6.35
CHEMBL3577880 1 6.08
CHEMBL3577882 1 6.02
CHEMBL3577890 1 5.94
CHEMBL3577895 1 5.79
CHEMBL3577891 1 5.48
CHEMBL3577884 1 5.44
CHEMBL3577901 1 5.21
CHEMBL3577879 1 -
CHEMBL3577887 1 -
CHEMBL3577888 1 -
CHEMBL3577892 1 -
CHEMBL3577894 1 -
CHEMBL3577896 1 -
CHEMBL3577897 1 -
CHEMBL3577899 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3577904 IC50 = 60.0 nM 7.22
CHEMBL3577902 IC50 = 62.0 nM 7.21
CHEMBL3577889 IC50 = 90.0 nM 7.05
CHEMBL3577881 IC50 = 94.0 nM 7.03
CHEMBL3577905 IC50 = 123.0 nM 6.91
CHEMBL3577883 IC50 = 145.0 nM 6.84
CHEMBL3577885 PF-05105679 IC50 = 181.0 nM 6.74
CHEMBL3577907 IC50 = 190.0 nM 6.72
CHEMBL3577900 IC50 = 202.0 nM 6.70
CHEMBL3577906 IC50 = 234.0 nM 6.63
CHEMBL3577898 IC50 = 288.0 nM 6.54
CHEMBL3576881 IC50 = 307.0 nM 6.51
CHEMBL3577903 IC50 = 328.0 nM 6.48
CHEMBL3577886 IC50 = 340.0 nM 6.47
CHEMBL3577893 IC50 = 446.0 nM 6.35
CHEMBL3577880 IC50 = 837.0 nM 6.08
CHEMBL3577882 IC50 = 951.0 nM 6.02
CHEMBL3577890 IC50 = 1159.0 nM 5.94
CHEMBL3577895 IC50 = 1613.0 nM 5.79
CHEMBL3577891 IC50 = 3282.0 nM 5.48
CHEMBL3577884 IC50 = 3619.0 nM 5.44
CHEMBL3577901 IC50 = 6104.0 nM 5.21
CHEMBL3577879 IC50 > 10000.0 nM -
CHEMBL3577887 IC50 > 6166.0 nM -
CHEMBL3577888 IC50 > 5000.0 nM -
CHEMBL3577892 IC50 > 7820.0 nM -
CHEMBL3577894 IC50 > 3249.0 nM -
CHEMBL3577896 IC50 > 9356.0 nM -
CHEMBL3577897 IC50 > 6863.0 nM -
CHEMBL3577899 IC50 > 10000.0 nM -