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Assay Detail

CHEMBL3579416

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HIV1 protease PR20 mutant expressed in Escherichia coli BL21 (DE3) incubated for 5 mins followed by Abz-Thr-Ile-Nle-p-nitro-Phe-GlnArg-NH2 substrate addition and measured over 5 mins by microplate reader based assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Human immunodeficiency virus 1
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Substituted Bis-THF Protease Inhibitors with Improved Potency against Highly Resistant Mature HIV-1 Protease PR20.
Agniswamy J, Louis JM, Shen CH, Yashchuk S, Ghosh AK, Weber IT.
J Med Chem (2015) 58 : 5088 -5095
PubMed 26010498 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 2 8.57 8.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3577575 1 8.77
CHEMBL3577576 1 8.37

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3577575 Ki = 1.7 nM 8.77
CHEMBL3577576 Ki = 4.3 nM 8.37