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Assay Detail

CHEMBL3579968

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human placental HexB using MUGS substrate incubated for 1 to 2 hrs at pH 7.0 by spectrofluorometry
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-hexosaminidase subunit beta (CHEMBL5877)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Pyrimethamine Derivatives: Insight into Binding Mechanism and Improved Enhancement of Mutant β-N-acetylhexosaminidase Activity.
Tropak MB, Zhang J, Yonekawa S, Rigat BA, Aulakh VS, Smith MR, Hwang HJ, Ciufolini MA, Mahuran DJ.
J Med Chem (2015) 58 : 4483 -4493
PubMed 25984755 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 5.09 5.35

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL36 PYRIMETHAMINE 4.0 1 5.35
CHEMBL22148 1 4.82

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL36 PYRIMETHAMINE IC50 = 4500.0 nM 5.35
CHEMBL22148 IC50 = 15000.0 nM 4.82