Assay Detail
Binding
CHEMBL3583057
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of [3H]-Prazosin from human alpha-1D adrenergic receptor transfected in CHO cell membranes after 2 hrs by microplate scintillation counting analysis
10
Total Activities
6
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | CHO |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Discovery of Quinazoline-Based Fluorescent Probes to α1-Adrenergic Receptors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 7.84 | 8.66 |
| Ki | 4 | 8.19 | 9.40 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3582270 | — | — | 2 | 9.40 |
| CHEMBL597 | PHENTOLAMINE | 4.0 | 2 | 8.12 |
| CHEMBL3582271 | — | — | 2 | 7.68 |
| CHEMBL3582272 | — | — | 2 | 7.56 |
| CHEMBL3582269 | — | — | 1 | - |
| CHEMBL3582268 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3582270 | — | Ki | = | 0.4 | nM | 9.40 |
| CHEMBL3582270 | — | IC50 | = | 2.2 | nM | 8.66 |
| CHEMBL597 | PHENTOLAMINE | Ki | = | 7.6 | nM | 8.12 |
| CHEMBL597 | PHENTOLAMINE | IC50 | = | 12.5 | nM | 7.90 |
| CHEMBL3582271 | — | Ki | = | 20.7 | nM | 7.68 |
| CHEMBL3582272 | — | Ki | = | 27.5 | nM | 7.56 |
| CHEMBL3582271 | — | IC50 | = | 34.0 | nM | 7.47 |
| CHEMBL3582272 | — | IC50 | = | 45.2 | nM | 7.34 |
| CHEMBL3582269 | — | IC50 | < | 5.0 | nM | - |
| CHEMBL3582268 | — | IC50 | < | 5.0 | nM | - |