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Assay Detail

CHEMBL3583194

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HIV-1 integrase assessed as inhibition of 3'-processing activity using 32P-labeled DNA as substrate after 1 hr by gel-based assay in presence of Mg2+
39
Total Activities
39
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Human immunodeficiency virus 1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Human immunodeficiency virus type 1 integrase (CHEMBL3471)
Type SINGLE PROTEIN
Organism Human immunodeficiency virus 1

Publication

N-Substituted Quinolinonyl Diketo Acid Derivatives as HIV Integrase Strand Transfer Inhibitors and Their Activity against RNase H Function of Reverse Transcriptase.
Pescatori L, Métifiot M, Chung S, Masoaka T, Cuzzucoli Crucitti G, Messore A, Pupo G, Madia VN, Saccoliti F, Scipione L, Tortorella S, Di Leva FS, Cosconati S, Marinelli L, Novellino E, Le Grice SF, Pommier Y, Marchand C, Costi R, Di Santo R.
J Med Chem (2015) 58 : 4610 -4623
PubMed 25961960 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 39 5.19 6.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL497501 1 6.70
CHEMBL2236601 RDS-1984 1 6.16
CHEMBL3582052 1 5.97
CHEMBL3582057 1 5.92
CHEMBL3582070 1 5.76
CHEMBL3582056 1 5.40
CHEMBL2236599 1 5.34
CHEMBL204656 ELVITEGRAVIR 4.0 1 5.09
CHEMBL3582072 1 5.01
CHEMBL254316 RALTEGRAVIR 4.0 1 4.89
CHEMBL3582080 1 4.75
CHEMBL3582073 1 4.69
CHEMBL3582071 1 4.68
CHEMBL3582058 1 4.66
CHEMBL3582061 1 4.57
CHEMBL3582060 1 4.55
CHEMBL3582082 1 4.16
CHEMBL3582043 1 -
CHEMBL3582041 1 -
CHEMBL3582042 1 -
CHEMBL2236603 RDS-2161 1 -
CHEMBL3582040 1 -
CHEMBL2236602 1 -
CHEMBL498713 1 -
CHEMBL3582038 1 -
CHEMBL3582039 1 -
CHEMBL2236589 20, RDS-2163 1 -
CHEMBL2236598 RDS-1624 1 -
CHEMBL2236600 1 -
CHEMBL3582053 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL497501 IC50 = 200.0 nM 6.70
CHEMBL2236601 RDS-1984 IC50 = 700.0 nM 6.16
CHEMBL3582052 IC50 = 1080.0 nM 5.97
CHEMBL3582057 IC50 = 1200.0 nM 5.92
CHEMBL3582070 IC50 = 1750.0 nM 5.76
CHEMBL3582056 IC50 = 4000.0 nM 5.40
CHEMBL2236599 IC50 = 4600.0 nM 5.34
CHEMBL204656 ELVITEGRAVIR IC50 = 8100.0 nM 5.09
CHEMBL3582072 IC50 = 9800.0 nM 5.01
CHEMBL254316 RALTEGRAVIR IC50 = 12800.0 nM 4.89
CHEMBL3582080 IC50 = 18000.0 nM 4.75
CHEMBL3582073 IC50 = 20500.0 nM 4.69
CHEMBL3582071 IC50 = 21000.0 nM 4.68
CHEMBL3582058 IC50 = 22000.0 nM 4.66
CHEMBL3582061 IC50 = 27000.0 nM 4.57
CHEMBL3582060 IC50 = 28000.0 nM 4.55
CHEMBL3582082 IC50 = 70000.0 nM 4.16
CHEMBL3582081 IC50 > 1000000.0 nM -
CHEMBL3582079 IC50 > 333000.0 nM -
CHEMBL3582078 IC50 > 1000000.0 nM -
CHEMBL3582077 IC50 > 333000.0 nM -
CHEMBL3582076 IC50 > 333000.0 nM -
CHEMBL3582075 IC50 > 333000.0 nM -
CHEMBL3582059 IC50 > 37000.0 nM -
CHEMBL2236588 IC50 > 4100.0 nM -
CHEMBL3582054 IC50 > 333000.0 nM -
CHEMBL3582053 IC50 = 266000.0 nM -
CHEMBL2236600 IC50 > 12300.0 nM -
CHEMBL2236598 RDS-1624 IC50 > 12300.0 nM -
CHEMBL2236589 20, RDS-2163 IC50 > 333000.0 nM -
CHEMBL3582039 IC50 = 125000.0 nM -
CHEMBL3582038 IC50 > 1000000.0 nM -
CHEMBL498713 IC50 > 50000.0 nM -
CHEMBL2236602 IC50 > 333000.0 nM -
CHEMBL3582040 IC50 > 37000.0 nM -
CHEMBL2236603 RDS-2161 IC50 > 4100.0 nM -
CHEMBL3582042 IC50 > 37000.0 nM -
CHEMBL3582041 IC50 > 333000.0 nM -
CHEMBL3582043 IC50 > 37000.0 nM -