Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3583540

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to biotinylated human recombinant glucokinase expressed in Escherichia coli assessed as dissociation rate constant by SPR method
17
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Hexokinase-4 (CHEMBL3820)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimizing glucokinase activator binding kinetics to lower in vivo hypoglycemia risk
Borzilleri KA, Pfefferkorn JA, Guzman-Perez A, Liu S, Qiu X, Chrunyk BA, Song X, Tu M, Filipski KJ, Aiello R, Derksen DR, Bourbonais FJ, Landro J, Bourassa P, D'Aquila T, Baker L, Barrucci N, Litchfield J, Atkinson K, Rolph TP, Withka JM
Medchemcomm (2014) 5 : 802 -807
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 17 7.35 8.67

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3217925 1 8.67
CHEMBL3580738 2 8.62
CHEMBL3580737 MK-0941 FREE BASE 2.0 1 8.53
CHEMBL3219124 AZD-1656 2.0 1 7.97
CHEMBL3580740 1 7.76
CHEMBL3580742 1 7.67
CHEMBL499520 1 7.59
CHEMBL3580739 1 7.06
CHEMBL3218477 1 7.05
CHEMBL2204663 1 6.94
CHEMBL3580741 1 6.85
CHEMBL608393 1 6.78
CHEMBL2165616 1 6.76
CHEMBL2165615 NERIGLIATIN 2.0 1 6.72
CHEMBL1783734 PIRAGLIATIN 2.0 1 6.66
CHEMBL2204655 1 6.37

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3217925 Kd = 2.16 nM 8.67
CHEMBL3580738 Kd = 2.42 nM 8.62
CHEMBL3580737 MK-0941 FREE BASE Kd = 2.93 nM 8.53
CHEMBL3219124 AZD-1656 Kd = 10.8 nM 7.97
CHEMBL3580740 Kd = 17.5 nM 7.76
CHEMBL3580742 Kd = 21.4 nM 7.67
CHEMBL499520 Kd = 25.6 nM 7.59
CHEMBL3580739 Kd = 86.4 nM 7.06
CHEMBL3218477 Kd = 89.9 nM 7.05
CHEMBL3580738 Kd = 96.8 nM 7.01
CHEMBL2204663 Kd = 114.1 nM 6.94
CHEMBL3580741 Kd = 141.5 nM 6.85
CHEMBL608393 Kd = 164.9 nM 6.78
CHEMBL2165616 Kd = 174.8 nM 6.76
CHEMBL2165615 NERIGLIATIN Kd = 189.2 nM 6.72
CHEMBL1783734 PIRAGLIATIN Kd = 218.4 nM 6.66
CHEMBL2204655 Kd = 427.3 nM 6.37