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Assay Detail

CHEMBL3583541

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Activation of recombinant human glucokinase assessed as formation of glucose-6-phosphate by G6PDH/NADP coupled assay
17
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Hexokinase-4 (CHEMBL3820)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimizing glucokinase activator binding kinetics to lower in vivo hypoglycemia risk
Borzilleri KA, Pfefferkorn JA, Guzman-Perez A, Liu S, Qiu X, Chrunyk BA, Song X, Tu M, Filipski KJ, Aiello R, Derksen DR, Bourbonais FJ, Landro J, Bourassa P, D'Aquila T, Baker L, Barrucci N, Litchfield J, Atkinson K, Rolph TP, Withka JM
Medchemcomm (2014) 5 : 802 -807
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 17 7.19 8.03

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3580738 2 8.03
CHEMBL3580737 MK-0941 FREE BASE 2.0 1 8.02
CHEMBL3217925 1 7.79
CHEMBL3580740 1 7.62
CHEMBL3218477 1 7.58
CHEMBL3219124 AZD-1656 2.0 1 7.55
CHEMBL499520 1 7.43
CHEMBL3580742 1 7.28
CHEMBL608393 1 7.10
CHEMBL2204663 1 6.94
CHEMBL3580739 1 6.92
CHEMBL2165615 NERIGLIATIN 2.0 1 6.76
CHEMBL3580741 1 6.72
CHEMBL2165616 1 6.68
CHEMBL1783734 PIRAGLIATIN 2.0 1 6.44
CHEMBL2204655 1 6.41

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3580738 EC50 = 9.4 nM 8.03
CHEMBL3580737 MK-0941 FREE BASE EC50 = 9.5 nM 8.02
CHEMBL3217925 EC50 = 16.2 nM 7.79
CHEMBL3580740 EC50 = 24.0 nM 7.62
CHEMBL3218477 EC50 = 26.0 nM 7.58
CHEMBL3219124 AZD-1656 EC50 = 28.0 nM 7.55
CHEMBL499520 EC50 = 37.0 nM 7.43
CHEMBL3580742 EC50 = 52.0 nM 7.28
CHEMBL608393 EC50 = 80.0 nM 7.10
CHEMBL2204663 EC50 = 114.0 nM 6.94
CHEMBL3580738 EC50 = 120.0 nM 6.92
CHEMBL3580739 EC50 = 121.0 nM 6.92
CHEMBL2165615 NERIGLIATIN EC50 = 174.0 nM 6.76
CHEMBL3580741 EC50 = 191.0 nM 6.72
CHEMBL2165616 EC50 = 210.0 nM 6.68
CHEMBL1783734 PIRAGLIATIN EC50 = 364.0 nM 6.44
CHEMBL2204655 EC50 = 391.0 nM 6.41