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Assay Detail

CHEMBL3587003

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of rat recombinant nNOS expressed in Escherichia coli by oxyhemoglobin NO assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nitric oxide synthase 1 (CHEMBL3048)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

2-Aminopyridines with a Truncated Side Chain To Improve Human Neuronal Nitric Oxide Synthase Inhibitory Potency and Selectivity.
Kang S, Li H, Tang W, Martásek P, Roman LJ, Poulos TL, Silverman RB.
J Med Chem (2015) 58 : 5548 -5560
PubMed 26120733 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 9 7.19 7.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3586661 1 7.77
CHEMBL3586664 1 7.62
CHEMBL3586663 1 7.47
CHEMBL3586660 1 7.46
CHEMBL3586658 1 7.21
CHEMBL3586662 1 7.17
CHEMBL3586656 1 6.89
CHEMBL3586657 1 6.72
CHEMBL3586659 1 6.37

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3586661 Ki = 17.0 nM 7.77
CHEMBL3586664 Ki = 24.0 nM 7.62
CHEMBL3586663 Ki = 34.0 nM 7.47
CHEMBL3586660 Ki = 35.0 nM 7.46
CHEMBL3586658 Ki = 62.0 nM 7.21
CHEMBL3586662 Ki = 67.0 nM 7.17
CHEMBL3586656 Ki = 130.0 nM 6.89
CHEMBL3586657 Ki = 190.0 nM 6.72
CHEMBL3586659 Ki = 430.0 nM 6.37