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Assay Detail

CHEMBL3587853

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human HIF1alpha-mediated transcription expressed in human U251 cells co-transfected with pGL2-TK-HRE incubated for 24 hrs by luciferase reporter gene assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type U-251
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Hypoxia-inducible factor 1-alpha (CHEMBL4261)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Tetracycline analogues with a selective inhibitory effect on HIF-1
Bendiabdellah Y, Rahman KM, Uranchimeg B, Nahar KS, Antonow D, Shoemaker RH, Melillo G, Zinzalla G, Thurston DE
Medchemcomm (2014) 5 : 923 -926
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 8 5.63 7.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL84 TOPOTECAN 4.0 1 7.92
CHEMBL3585463 1 5.85
CHEMBL3585448 1 5.64
CHEMBL3585467 1 5.39
CHEMBL3585464 1 5.17
CHEMBL3585447 1 5.17
CHEMBL3585466 1 5.11
CHEMBL3585465 1 4.81

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL84 TOPOTECAN EC50 = 12.0 nM 7.92
CHEMBL3585463 EC50 = 1400.0 nM 5.85
CHEMBL3585448 EC50 = 2300.0 nM 5.64
CHEMBL3585467 EC50 = 4100.0 nM 5.39
CHEMBL3585464 EC50 = 6700.0 nM 5.17
CHEMBL3585447 EC50 = 6700.0 nM 5.17
CHEMBL3585466 EC50 = 7800.0 nM 5.11
CHEMBL3585465 EC50 = 15500.0 nM 4.81