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Assay Detail

CHEMBL3593134

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of MEK U911-activated ERK2 (unknown origin) using Erktide as substrate preincubated with enzyme for 20 mins prior to substrate addition by RapidFire Mass Spectrometry in presence of ATP at Km concentration
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mitogen-activated protein kinase 1 (CHEMBL4040)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Guided Design of Highly Selective and Potent Covalent Inhibitors of ERK1/2.
Ward RA, Colclough N, Challinor M, Debreczeni JE, Eckersley K, Fairley G, Feron L, Flemington V, Graham MA, Greenwood R, Hopcroft P, Howard TD, James M, Jones CD, Jones CR, Renshaw J, Roberts K, Snow L, Tonge M, Yeung K.
J Med Chem (2015) 58 : 4790 -4801
PubMed 25977981 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 7.11 8.51

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3590120 1 8.51
CHEMBL3590121 1 8.16
CHEMBL3590110 1 8.12
CHEMBL3590118 1 8.07
CHEMBL3590123 1 8.00
CHEMBL3590125 1 7.41
CHEMBL3590116 1 7.40
CHEMBL3590117 1 7.38
CHEMBL3590114 1 7.36
CHEMBL3590111 1 7.13
CHEMBL3590122 1 7.11
CHEMBL3590124 1 7.07
CHEMBL3590115 1 6.58
CHEMBL3590113 1 6.55
CHEMBL3590109 1 6.43
CHEMBL3590119 1 6.32
CHEMBL3590108 1 5.29
CHEMBL3590112 1 5.05
CHEMBL3590107 1 -
CHEMBL3590106 ULIXERTINIB 2.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3590120 IC50 = 3.1 nM 8.51
CHEMBL3590121 IC50 = 6.9 nM 8.16
CHEMBL3590110 IC50 = 7.5 nM 8.12
CHEMBL3590118 IC50 = 8.5 nM 8.07
CHEMBL3590123 IC50 = 9.9 nM 8.00
CHEMBL3590125 IC50 = 39.0 nM 7.41
CHEMBL3590116 IC50 = 40.0 nM 7.40
CHEMBL3590117 IC50 = 42.0 nM 7.38
CHEMBL3590114 IC50 = 44.0 nM 7.36
CHEMBL3590111 IC50 = 74.0 nM 7.13
CHEMBL3590122 IC50 = 77.0 nM 7.11
CHEMBL3590124 IC50 = 85.0 nM 7.07
CHEMBL3590115 IC50 = 260.0 nM 6.58
CHEMBL3590113 IC50 = 280.0 nM 6.55
CHEMBL3590109 IC50 = 370.0 nM 6.43
CHEMBL3590119 IC50 = 480.0 nM 6.32
CHEMBL3590108 IC50 = 5100.0 nM 5.29
CHEMBL3590112 IC50 = 9000.0 nM 5.05
CHEMBL3590107 IC50 < 0.3 nM -
CHEMBL3590106 ULIXERTINIB IC50 < 0.3 nM -