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Assay Detail

CHEMBL3594729

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant GluN1/GluN2B receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of glutamate-evoked current by whole-cell voltage-clamp method
17
Total Activities
16
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Glutamate NMDA receptor; GRIN1/GRIN2B (CHEMBL1907603)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

A New Class of Potent N-Methyl-D-Aspartate Receptor Inhibitors: Sulfated Neuroactive Steroids with Lipophilic D-Ring Modifications.
Kudova E, Chodounska H, Slavikova B, Budesinsky M, Nekardova M, Vyklicky V, Krausova B, Svehla P, Vyklicky L.
J Med Chem (2015) 58 : 5950 -5966
PubMed 26171651 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 5.98 7.05
Inhibition 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3593892 1 7.05
CHEMBL3594108 1 6.77
CHEMBL3594106 1 6.40
CHEMBL3594107 1 6.30
CHEMBL3594112 1 6.22
CHEMBL3594109 1 6.22
CHEMBL3594117 1 6.16
CHEMBL3594113 1 6.10
CHEMBL3594116 1 6.05
CHEMBL3594115 1 5.96
CHEMBL3594110 1 5.92
CHEMBL3594111 1 5.80
CHEMBL3594118 1 5.66
CHEMBL3594120 1 5.27
CHEMBL3594114 1 5.16
CHEMBL1179566 2 4.63

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3593892 IC50 = 90.0 nM 7.05
CHEMBL3594108 IC50 = 170.0 nM 6.77
CHEMBL3594106 IC50 = 400.0 nM 6.40
CHEMBL3594107 IC50 = 500.0 nM 6.30
CHEMBL3594112 IC50 = 600.0 nM 6.22
CHEMBL3594109 IC50 = 600.0 nM 6.22
CHEMBL3594117 IC50 = 700.0 nM 6.16
CHEMBL3594113 IC50 = 800.0 nM 6.10
CHEMBL3594116 IC50 = 900.0 nM 6.05
CHEMBL3594115 IC50 = 1100.0 nM 5.96
CHEMBL3594110 IC50 = 1200.0 nM 5.92
CHEMBL3594111 IC50 = 1600.0 nM 5.80
CHEMBL3594118 IC50 = 2200.0 nM 5.66
CHEMBL3594120 IC50 = 5400.0 nM 5.27
CHEMBL3594114 IC50 = 7000.0 nM 5.16
CHEMBL1179566 IC50 = 23400.0 nM 4.63
CHEMBL1179566 Inhibition - % -