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Assay Detail

CHEMBL3595191

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant GST-tagged Aurora-A kinase domain (S123 to S401) (unknown origin) expressed in insect Sf9 cells using tetra(LRRASLG) peptide as substrate after 90 mins by Kinase-Glo assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Aurora kinase A (CHEMBL4722)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of a potent 5-phenyl-thiazol-2-ylamine-based inhibitor of FLT3 with activity against drug resistance-conferring point mutations.
Chen CT, Hsu JT, Lin WH, Lu CT, Yen SC, Hsu T, Huang YL, Song JS, Chen CH, Chou LH, Yen KJ, Chen CP, Kuo PC, Huang CL, Liu HE, Chao YS, Yeh TK, Jiaang WT.
Eur J Med Chem (2015) 100 : 151 -161
PubMed 26081023 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 7.04 7.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3593296 1 7.60
CHEMBL3593290 1 7.50
CHEMBL3593292 1 7.44
CHEMBL3593291 1 7.42
CHEMBL3593289 1 7.42
CHEMBL3593288 1 7.39
CHEMBL3593293 1 7.37
CHEMBL3593287 1 7.25
CHEMBL608533 MIDOSTAURIN 4.0 1 7.10
CHEMBL3593285 1 7.02
CHEMBL3593295 1 6.85
CHEMBL3593284 1 6.82
CHEMBL3593286 1 6.57
CHEMBL3593294 1 6.50
CHEMBL1336 SORAFENIB 4.0 1 5.42
CHEMBL576982 QUIZARTINIB 4.0 1 -
CHEMBL3593283 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3593296 IC50 = 25.0 nM 7.60
CHEMBL3593290 IC50 = 32.0 nM 7.50
CHEMBL3593292 IC50 = 36.0 nM 7.44
CHEMBL3593291 IC50 = 38.0 nM 7.42
CHEMBL3593289 IC50 = 38.0 nM 7.42
CHEMBL3593288 IC50 = 41.0 nM 7.39
CHEMBL3593293 IC50 = 43.0 nM 7.37
CHEMBL3593287 IC50 = 56.0 nM 7.25
CHEMBL608533 MIDOSTAURIN IC50 = 80.0 nM 7.10
CHEMBL3593285 IC50 = 95.0 nM 7.02
CHEMBL3593295 IC50 = 140.0 nM 6.85
CHEMBL3593284 IC50 = 150.0 nM 6.82
CHEMBL3593286 IC50 = 270.0 nM 6.57
CHEMBL3593294 IC50 = 320.0 nM 6.50
CHEMBL1336 SORAFENIB IC50 = 3800.0 nM 5.42
CHEMBL576982 QUIZARTINIB IC50 > 10000.0 nM -
CHEMBL3593283 IC50 > 10000.0 nM -