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Assay Detail

CHEMBL3595596

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HIV1 recombinant wild type reverse transcriptase p66/p51 assessed as reduction in biotin deoxyuridine triphosphate (biotin-dUTP) incorporation using poly(rA)/oligo (dT)16 template and primer by spectrofluorometry
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Human immunodeficiency virus 1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Human immunodeficiency virus type 1 reverse transcriptase (CHEMBL247)
Type SINGLE PROTEIN
Organism Human immunodeficiency virus 1

Publication

Hybrid chemistry. Part 4: Discovery of etravirine-VRX-480773 hybrids as potent HIV-1 non-nucleoside reverse transcriptase inhibitors.
Wan ZY, Tao Y, Wang YF, Mao TQ, Yin H, Chen FE, Piao HR, De Clercq E, Daelemans D, Pannecouque C.
Bioorg Med Chem (2015) 23 : 4248 -4255
PubMed 26162497 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 6.31 7.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL223228 EFAVIRENZ 4.0 1 7.66
CHEMBL3593877 1 6.33
CHEMBL57 NEVIRAPINE 4.0 1 6.09
CHEMBL3593248 1 5.93
CHEMBL3593512 1 5.56

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL223228 EFAVIRENZ IC50 = 22.0 nM 7.66
CHEMBL3593877 IC50 = 473.0 nM 6.33
CHEMBL57 NEVIRAPINE IC50 = 807.0 nM 6.09
CHEMBL3593248 IC50 = 1169.0 nM 5.93
CHEMBL3593512 IC50 = 2775.0 nM 5.56