Assay Detail
Binding
CHEMBL3595970
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of Wistar rat brain Na(+)/K(+) ATPase alpha-2/3 assessed as amount of Pi release after 1 hr by colorimetric method
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Rattus norvegicus |
| Confidence | 7 — Homologous single protein target |
| Curated By | Autocuration |
Target
Sodium/potassium-transporting ATPase subunit alpha-2/alpha-3 (CHEMBL3885640) ↗| Type | PROTEIN COMPLEX |
| Organism | Rattus norvegicus |
Publication
γ-Benzylidene digoxin derivatives synthesis and molecular modeling: Evaluation of anticancer and the Na,K-ATPase activity effect.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 5.67 | 6.66 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1751 | DIGOXIN | 4.0 | 1 | 6.66 |
| CHEMBL3594276 | — | — | 1 | 6.34 |
| CHEMBL3594278 | — | — | 1 | 6.18 |
| CHEMBL3594280 | — | — | 1 | 5.40 |
| CHEMBL3594277 | — | — | 1 | 4.75 |
| CHEMBL3856118 | — | — | 1 | 4.70 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1751 | DIGOXIN | IC50 | = | 220.0 | nM | 6.66 |
| CHEMBL3594276 | — | IC50 | = | 460.0 | nM | 6.34 |
| CHEMBL3594278 | — | IC50 | = | 660.0 | nM | 6.18 |
| CHEMBL3594280 | — | IC50 | = | 4000.0 | nM | 5.40 |
| CHEMBL3594277 | — | IC50 | = | 18000.0 | nM | 4.75 |
| CHEMBL3856118 | — | IC50 | = | 20000.0 | nM | 4.70 |