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Assay Detail

CHEMBL3602283

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of MDM2/p53 interaction (unknown origin) using biotinylated MDM2 and Cy5-p53 (18 to 26 amino acids) by TR-FRET assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Tumour suppressor p53/oncoprotein Mdm2 (CHEMBL1907611)
Type PROTEIN-PROTEIN INTERACTION
Organism Homo sapiens

Publication

Discovery of a Dihydroisoquinolinone Derivative (NVP-CGM097): A Highly Potent and Selective MDM2 Inhibitor Undergoing Phase 1 Clinical Trials in p53wt Tumors.
Holzer P, Masuya K, Furet P, Kallen J, Valat-Stachyra T, Ferretti S, Berghausen J, Bouisset-Leonard M, Buschmann N, Pissot-Soldermann C, Rynn C, Ruetz S, Stutz S, Chène P, Jeay S, Gessier F.
J Med Chem (2015) 58 : 6348 -6358
PubMed 26181851 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 8.35 8.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3601323 1 8.96
CHEMBL3601399 1 8.80
CHEMBL3601397 1 8.80
CHEMBL3601398 CGM-097 1.0 1 8.77
CHEMBL3601396 1 8.77
CHEMBL3601322 1 8.72
CHEMBL3601402 1 8.70
CHEMBL3601317 1 8.64
CHEMBL3601401 1 8.60
CHEMBL3601321 1 8.52
CHEMBL3601320 1 8.52
CHEMBL3601319 1 8.40
CHEMBL191334 NUTLIN-3 1 8.39
CHEMBL3601403 1 8.12
CHEMBL3601316 1 8.10
CHEMBL3601400 1 7.94
CHEMBL3601324 1 7.70
CHEMBL3601318 1 5.93

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3601323 IC50 = 1.1 nM 8.96
CHEMBL3601399 IC50 = 1.6 nM 8.80
CHEMBL3601397 IC50 = 1.6 nM 8.80
CHEMBL3601398 CGM-097 IC50 = 1.7 nM 8.77
CHEMBL3601396 IC50 = 1.7 nM 8.77
CHEMBL3601322 IC50 = 1.9 nM 8.72
CHEMBL3601402 IC50 = 2.0 nM 8.70
CHEMBL3601317 IC50 = 2.3 nM 8.64
CHEMBL3601401 IC50 = 2.5 nM 8.60
CHEMBL3601321 IC50 = 3.0 nM 8.52
CHEMBL3601320 IC50 = 3.0 nM 8.52
CHEMBL3601319 IC50 = 4.0 nM 8.40
CHEMBL191334 NUTLIN-3 IC50 = 4.1 nM 8.39
CHEMBL3601403 IC50 = 7.6 nM 8.12
CHEMBL3601316 IC50 = 8.0 nM 8.10
CHEMBL3601400 IC50 = 11.4 nM 7.94
CHEMBL3601324 IC50 = 20.0 nM 7.70
CHEMBL3601318 IC50 = 1171.0 nM 5.93