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Assay Detail

CHEMBL3603305

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at rat alpha-7 nicotinic acetylcholine receptor expressed in Xenopus oocytes assessed as inhibition of acetylcholine-induced currents preincubated for 10 mins followed by nicotine induction by two-electrode voltage-clamp electrophysiology assay
8
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor subunit alpha-7 (CHEMBL4980)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Pharmacological profile of zacopride and new quaternarized fluorobenzamide analogues on mammalian α7 nicotinic acetylcholine receptor.
Bourdin CM, Lebreton J, Mathé-Allainmat M, Thany SH.
Bioorg Med Chem Lett (2015) 25 : 3184 -3188
PubMed 26087938 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 4.80 5.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL18041 ZACOPRIDE 2.0 2 5.15
CHEMBL3600666 2 5.09
CHEMBL3600667 2 4.55
CHEMBL3600668 2 4.41

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL18041 ZACOPRIDE IC50 = 7079.46 nM 5.15
CHEMBL18041 ZACOPRIDE IC50 = 7040.0 nM 5.15
CHEMBL3600666 IC50 = 8128.31 nM 5.09
CHEMBL3600666 IC50 = 8070.0 nM 5.09
CHEMBL3600667 IC50 = 28183.83 nM 4.55
CHEMBL3600667 IC50 = 28400.0 nM 4.55
CHEMBL3600668 IC50 = 38904.51 nM 4.41
CHEMBL3600668 IC50 = 39300.0 nM 4.41