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Assay Detail

CHEMBL3606492

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity against human NCI-H460 cells assessed as inhibition of cell proliferation/survival after 72 hrs by resazurin dye reduction assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type NCI-H460
Confidence 1 — Organism
Curated By Autocuration

Target

NCI-H460 (CHEMBL396)
Type CELL-LINE
Organism Homo sapiens

Publication

Discovery of Potent 17β-Hydroxywithanolides for Castration-Resistant Prostate Cancer by High-Throughput Screening of a Natural Products Library for Androgen-Induced Gene Expression Inhibitors.
Xu YM, Liu MX, Grunow N, Wijeratne EM, Paine-Murrieta G, Felder S, Kris RM, Gunatilaka AA.
J Med Chem (2015) 58 : 6984 -6993
PubMed 26305181 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 6.02 7.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL53463 DOXORUBICIN 4.0 1 7.30
CHEMBL3605565 1 6.10
CHEMBL3605564 1 5.92
CHEMBL3605562 1 5.70
CHEMBL3605566 1 5.68
CHEMBL3605560 1 5.41
CHEMBL3605561 1 -
CHEMBL3605563 1 -
CHEMBL517080 WITHAFERIN A 1.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL53463 DOXORUBICIN IC50 = 50.0 nM 7.30
CHEMBL3605565 IC50 = 800.0 nM 6.10
CHEMBL3605564 IC50 = 1200.0 nM 5.92
CHEMBL3605562 IC50 = 2000.0 nM 5.70
CHEMBL3605566 IC50 = 2100.0 nM 5.68
CHEMBL3605560 IC50 = 3900.0 nM 5.41
CHEMBL3605561 IC50 > 5000.0 nM -
CHEMBL3605563 IC50 > 5000.0 nM -
CHEMBL517080 WITHAFERIN A IC50 < 400.0 nM -