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Assay Detail

CHEMBL3606871

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of SUMO-tagged ULK1 (unknown origin) kinase domain expressed in KRX cells using [32P]-gamma-ATP and myelin basic protein substrate incubated for 7 mins
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type KRX
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase ULK1 (CHEMBL6006)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and structure of a new inhibitor scaffold of the autophagy initiating kinase ULK1.
Lazarus MB, Shokat KM.
Bioorg Med Chem (2015) 23 : 5483 -5488
PubMed 26275681 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 7.24 8.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3605055 1 8.28
CHEMBL3603853 1 7.17
CHEMBL577784 BX-795 1 7.06
CHEMBL3605056 1 6.92
CHEMBL3605057 1 6.77

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3605055 IC50 = 5.3 nM 8.28
CHEMBL3603853 IC50 = 67.0 nM 7.17
CHEMBL577784 BX-795 IC50 = 87.0 nM 7.06
CHEMBL3605056 IC50 = 120.0 nM 6.92
CHEMBL3605057 IC50 = 170.0 nM 6.77