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Assay Detail

CHEMBL3607347

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to full length recombinant human PARP-2 by fluorescence polarization displacement assay
41
Total Activities
41
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Poly [ADP-ribose] polymerase 2 (CHEMBL5366)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 2-[1-(4,4-Difluorocyclohexyl)piperidin-4-yl]-6-fluoro-3-oxo-2,3-dihydro-1H-isoindole-4-carboxamide (NMS-P118): A Potent, Orally Available, and Highly Selective PARP-1 Inhibitor for Cancer Therapy.
Papeo G, Posteri H, Borghi D, Busel AA, Caprera F, Casale E, Ciomei M, Cirla A, Corti E, D'Anello M, Fasolini M, Forte B, Galvani A, Isacchi A, Khvat A, Krasavin MY, Lupi R, Orsini P, Perego R, Pesenti E, Pezzetta D, Rainoldi S, Riccardi-Sirtori F, Scolaro A, Sola F, Zuccotto F, Felder ER, Donati D, Montagnoli A.
J Med Chem (2015) 58 : 6875 -6898
PubMed 26222319 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 41 5.93 7.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3605990 1 7.48
CHEMBL3605992 1 7.12
CHEMBL3605967 1 6.90
CHEMBL3605972 1 6.75
CHEMBL3605993 1 6.58
CHEMBL3605973 1 6.54
CHEMBL3605991 1 6.47
CHEMBL3605982 1 6.38
CHEMBL1329044 1 6.38
CHEMBL3605994 1 6.36
CHEMBL3605970 1 6.24
CHEMBL3606012 1 6.23
CHEMBL3605981 1 6.17
CHEMBL3606013 1 6.01
CHEMBL3605984 1 5.95
CHEMBL3606009 1 5.84
CHEMBL3606011 1 5.77
CHEMBL3606000 1 5.64
CHEMBL3606001 1 5.62
CHEMBL3606015 1 5.60
CHEMBL3605985 1 5.58
CHEMBL3606016 1 5.52
CHEMBL3605978 1 5.47
CHEMBL3606004 1 5.44
CHEMBL3606010 1 5.43
CHEMBL3606018 1 5.41
CHEMBL3605983 1 5.24
CHEMBL3605976 1 5.23
CHEMBL3606007 1 5.17
CHEMBL3605999 1 5.16

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3605990 Kd = 33.0 nM 7.48
CHEMBL3605992 Kd = 75.0 nM 7.12
CHEMBL3605967 Kd = 125.0 nM 6.90
CHEMBL3605972 Kd = 178.0 nM 6.75
CHEMBL3605993 Kd = 264.0 nM 6.58
CHEMBL3605973 Kd = 286.0 nM 6.54
CHEMBL3605991 Kd = 340.0 nM 6.47
CHEMBL3605982 Kd = 420.0 nM 6.38
CHEMBL1329044 Kd = 422.0 nM 6.38
CHEMBL3605994 Kd = 441.0 nM 6.36
CHEMBL3605970 Kd = 575.0 nM 6.24
CHEMBL3606012 Kd = 583.0 nM 6.23
CHEMBL3605981 Kd = 670.0 nM 6.17
CHEMBL3606013 Kd = 983.0 nM 6.01
CHEMBL3605984 Kd = 1112.0 nM 5.95
CHEMBL3606009 Kd = 1435.0 nM 5.84
CHEMBL3606011 Kd = 1698.0 nM 5.77
CHEMBL3606000 Kd = 2289.0 nM 5.64
CHEMBL3606001 Kd = 2425.0 nM 5.62
CHEMBL3606015 Kd = 2510.0 nM 5.60
CHEMBL3605985 Kd = 2600.0 nM 5.58
CHEMBL3606016 Kd = 3017.0 nM 5.52
CHEMBL3605978 Kd = 3360.0 nM 5.47
CHEMBL3606004 Kd = 3627.0 nM 5.44
CHEMBL3606010 Kd = 3700.0 nM 5.43
CHEMBL3606018 Kd = 3937.0 nM 5.41
CHEMBL3605983 Kd = 5776.0 nM 5.24
CHEMBL3605976 Kd = 5870.0 nM 5.23
CHEMBL3606007 Kd = 6804.0 nM 5.17
CHEMBL3605999 Kd = 6916.0 nM 5.16
CHEMBL3605997 Kd = 8714.0 nM 5.06
CHEMBL3605998 Kd = 8991.0 nM 5.05
CHEMBL3606003 Kd > 10000.0 nM -
CHEMBL3606005 Kd > 10000.0 nM -
CHEMBL3606006 Kd > 10000.0 nM -
CHEMBL3606008 Kd > 10000.0 nM -
CHEMBL3605996 Kd > 10000.0 nM -
CHEMBL3605974 Kd < 30.0 nM -
CHEMBL3605986 Kd > 10000.0 nM -
CHEMBL3606017 Kd > 10000.0 nM -
CHEMBL3606014 Kd > 10000.0 nM -