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Assay Detail

CHEMBL3607383

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inverse agonist activity at APC-labeled RORgammat-LBD (unknown origin) assessed as inhibition of europium-labeled SRC1 recruitment after 1 hr by dual FRET analysis
30
Total Activities
15
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nuclear receptor ROR-gamma (CHEMBL1741186)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of N-(4-aryl-5-aryloxy-thiazol-2-yl)-amides as potent RORγt inverse agonists.
Wang Y, Yang T, Liu Q, Ma Y, Yang L, Zhou L, Xiang Z, Cheng Z, Lu S, Orband-Miller LA, Zhang W, Wu Q, Zhang K, Li Y, Xiang JN, Elliott JD, Leung S, Ren F, Lin X.
Bioorg Med Chem (2015) 23 : 5293 -5302
PubMed 26277758 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 7.29 8.10
Imax 15 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3605084 2 8.10
CHEMBL3605081 2 7.90
CHEMBL3605088 2 7.90
CHEMBL3605090 2 7.90
CHEMBL3605082 2 7.80
CHEMBL3605085 2 7.70
CHEMBL3605086 2 7.70
CHEMBL3605092 2 7.70
CHEMBL3314014 2 7.70
CHEMBL3605089 2 7.50
CHEMBL3605087 2 7.40
CHEMBL3605091 2 6.40
CHEMBL3105815 2 6.00
CHEMBL3605078 2 5.90
CHEMBL3605077 2 5.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3605084 IC50 = 7.943 nM 8.10
CHEMBL3605081 IC50 = 12.59 nM 7.90
CHEMBL3605088 IC50 = 12.59 nM 7.90
CHEMBL3605090 IC50 = 12.59 nM 7.90
CHEMBL3605082 IC50 = 15.85 nM 7.80
CHEMBL3314014 IC50 = 19.95 nM 7.70
CHEMBL3605092 IC50 = 19.95 nM 7.70
CHEMBL3605085 IC50 = 19.95 nM 7.70
CHEMBL3605086 IC50 = 19.95 nM 7.70
CHEMBL3605089 IC50 = 31.62 nM 7.50
CHEMBL3605087 IC50 = 39.81 nM 7.40
CHEMBL3605091 IC50 = 398.11 nM 6.40
CHEMBL3105815 IC50 = 1000.0 nM 6.00
CHEMBL3605078 IC50 = 1258.93 nM 5.90
CHEMBL3605077 IC50 = 1995.26 nM 5.70
CHEMBL3105815 Imax = 91.0 % -
CHEMBL3605077 Imax = 58.0 % -
CHEMBL3605078 Imax = 76.0 % -
CHEMBL3605081 Imax = 58.0 % -
CHEMBL3605082 Imax = 52.0 % -
CHEMBL3605084 Imax = 31.0 % -
CHEMBL3605085 Imax = 60.0 % -
CHEMBL3605086 Imax = 45.0 % -
CHEMBL3605087 Imax = 57.0 % -
CHEMBL3605088 Imax = 52.0 % -
CHEMBL3605089 Imax = 53.0 % -
CHEMBL3605090 Imax = 40.0 % -
CHEMBL3605091 Imax = 85.0 % -
CHEMBL3605092 Imax = 79.0 % -
CHEMBL3314014 Imax = 61.0 % -