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Assay Detail

CHEMBL3607426

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EZH2 Y641N mutant (unknown origin) using biotinylated nucleosome, H3K27me3 activator and [3H]-SAM incubated for 60 mins by top-count based method
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone-lysine N-methyltransferase EZH2 (CHEMBL2189110)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery, design, and synthesis of indole-based EZH2 inhibitors.
Gehling VS, Vaswani RG, Nasveschuk CG, Duplessis M, Iyer P, Balasubramanian S, Zhao F, Good AC, Campbell R, Lee C, Dakin LA, Cook AS, Gagnon A, Harmange JC, Audia JE, Cummings RT, Normant E, Trojer P, Albrecht BK.
Bioorg Med Chem Lett (2015) 25 : 3644 -3649
PubMed 26189078 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 7.29 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3414621 TAZEMETOSTAT 4.0 1 9.00
CHEMBL3287735 GSK2816126 1.0 1 8.70
CHEMBL3605457 1 8.52
CHEMBL3605455 1 8.52
CHEMBL3605456 1 8.40
CHEMBL3605453 1 8.40
CHEMBL3605454 1 8.15
CHEMBL3605446 1 7.47
CHEMBL3605458 1 7.40
CHEMBL3605452 1 7.37
CHEMBL3605445 1 7.21
CHEMBL3605447 1 7.01
CHEMBL3605451 1 6.93
CHEMBL3605449 1 6.45
CHEMBL3605448 1 6.41
CHEMBL3605459 1 6.31
CHEMBL3605450 1 6.29
CHEMBL3605441 1 6.10
CHEMBL3605442 1 5.87
CHEMBL3605443 1 5.26
CHEMBL3603857 1 -
CHEMBL3605444 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3414621 TAZEMETOSTAT IC50 = 1.0 nM 9.00
CHEMBL3287735 GSK2816126 IC50 = 2.0 nM 8.70
CHEMBL3605457 IC50 = 3.0 nM 8.52
CHEMBL3605455 IC50 = 3.0 nM 8.52
CHEMBL3605456 IC50 = 4.0 nM 8.40
CHEMBL3605453 IC50 = 4.0 nM 8.40
CHEMBL3605454 IC50 = 7.0 nM 8.15
CHEMBL3605446 IC50 = 34.0 nM 7.47
CHEMBL3605458 IC50 = 40.0 nM 7.40
CHEMBL3605452 IC50 = 43.0 nM 7.37
CHEMBL3605445 IC50 = 62.0 nM 7.21
CHEMBL3605447 IC50 = 97.0 nM 7.01
CHEMBL3605451 IC50 = 118.0 nM 6.93
CHEMBL3605449 IC50 = 358.0 nM 6.45
CHEMBL3605448 IC50 = 388.0 nM 6.41
CHEMBL3605459 IC50 = 485.0 nM 6.31
CHEMBL3605450 IC50 = 512.0 nM 6.29
CHEMBL3605441 IC50 = 795.0 nM 6.10
CHEMBL3605442 IC50 = 1350.0 nM 5.87
CHEMBL3605443 IC50 = 5550.0 nM 5.26
CHEMBL3603857 IC50 > 10000.0 nM -
CHEMBL3605444 IC50 > 10000.0 nM -