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Assay Detail

CHEMBL3607773

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of p53-MDM2 interaction (unknown origin) by TR-FRET assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Tumour suppressor p53/oncoprotein Mdm2 (CHEMBL1907611)
Type PROTEIN-PROTEIN INTERACTION
Organism Homo sapiens

Publication

Discovery of dihydroisoquinolinone derivatives as novel inhibitors of the p53-MDM2 interaction with a distinct binding mode.
Gessier F, Kallen J, Jacoby E, Chène P, Stachyra-Valat T, Ruetz S, Jeay S, Holzer P, Masuya K, Furet P.
Bioorg Med Chem Lett (2015) 25 : 3621 -3625
PubMed 26141769 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 6.97 8.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3601317 1 8.64
CHEMBL3601316 1 8.10
CHEMBL191334 NUTLIN-3 1 8.00
CHEMBL3604664 1 7.82
CHEMBL3604663 1 6.92
CHEMBL3604661 1 6.92
CHEMBL3604662 1 6.77
CHEMBL3604660 1 6.42
CHEMBL3604658 1 6.27
CHEMBL3604659 1 6.00
CHEMBL3601318 1 5.92
CHEMBL3603849 1 5.85

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3601317 IC50 = 2.3 nM 8.64
CHEMBL3601316 IC50 = 8.0 nM 8.10
CHEMBL191334 NUTLIN-3 IC50 = 10.0 nM 8.00
CHEMBL3604664 IC50 = 15.0 nM 7.82
CHEMBL3604663 IC50 = 120.0 nM 6.92
CHEMBL3604661 IC50 = 120.0 nM 6.92
CHEMBL3604662 IC50 = 170.0 nM 6.77
CHEMBL3604660 IC50 = 380.0 nM 6.42
CHEMBL3604658 IC50 = 540.0 nM 6.27
CHEMBL3604659 IC50 = 1000.0 nM 6.00
CHEMBL3601318 IC50 = 1200.0 nM 5.92
CHEMBL3603849 IC50 = 1400.0 nM 5.85