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Assay Detail

CHEMBL3610437

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to PLK1 (unknown origin) expressed in HEK293 cells after 1 hr by proprietary competition assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase PLK1 (CHEMBL3024)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

BRD4 Structure-Activity Relationships of Dual PLK1 Kinase/BRD4 Bromodomain Inhibitor BI-2536.
Chen L, Yap JL, Yoshioka M, Lanning ME, Fountain RN, Raje M, Scheenstra JA, Strovel JW, Fletcher S.
ACS Med Chem Lett (2015) 6 : 764 -769
PubMed 26191363 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 18 8.65 9.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL513909 BI-2536 2.0 1 9.66
CHEMBL3609305 1 9.38
CHEMBL3609310 1 9.28
CHEMBL3609314 1 9.08
CHEMBL3609300 1 9.04
CHEMBL3609306 1 8.38
CHEMBL3609308 1 8.24
CHEMBL3609307 1 8.16
CHEMBL3609315 1 6.62
CHEMBL3609299 1 -
CHEMBL3609301 1 -
CHEMBL3609313 1 -
CHEMBL3609312 1 -
CHEMBL3609311 1 -
CHEMBL3609302 1 -
CHEMBL3609309 1 -
CHEMBL3609303 1 -
CHEMBL3609304 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL513909 BI-2536 Ki = 0.22 nM 9.66
CHEMBL3609305 Ki = 0.42 nM 9.38
CHEMBL3609310 Ki = 0.52 nM 9.28
CHEMBL3609314 Ki = 0.84 nM 9.08
CHEMBL3609300 Ki = 0.91 nM 9.04
CHEMBL3609306 Ki = 4.2 nM 8.38
CHEMBL3609308 Ki = 5.8 nM 8.24
CHEMBL3609307 Ki = 6.95 nM 8.16
CHEMBL3609315 Ki = 240.0 nM 6.62
CHEMBL3609299 Ki - -
CHEMBL3609301 Ki - -
CHEMBL3609313 Ki - -
CHEMBL3609312 Ki - -
CHEMBL3609311 Ki - -
CHEMBL3609302 Ki - -
CHEMBL3609309 Ki - -
CHEMBL3609303 Ki - -
CHEMBL3609304 Ki - -