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Assay Detail

CHEMBL3610666

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-epibatidine from alpha4beta2 nAChR in rat cerebral cortex homogenates after 4 hrs by liquid scintillation counting
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Cerebral cortex
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907596)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Synthesis, nicotinic acetylcholine receptor binding, in vitro and in vivo pharmacology properties of 3'-(substituted pyridinyl)-deschloroepibatidine analogs.
Ondachi PW, Ye Z, Castro AH, Luetje CW, Damaj MI, Mascarella SW, Navarro HA, Carroll FI.
Bioorg Med Chem (2015) 23 : 5693 -5701
PubMed 26233800 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 13 9.63 10.59

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL513692 1 10.59
CHEMBL3608514 1 10.03
CHEMBL3608516 1 9.92
CHEMBL1076903 VARENICLINE 4.0 1 9.92
CHEMBL243971 1 9.89
CHEMBL3608511 1 9.74
CHEMBL3608510 1 9.66
CHEMBL3608518 1 9.57
CHEMBL3608512 1 9.54
CHEMBL3608513 1 9.42
CHEMBL3608515 1 9.09
CHEMBL3608517 1 9.05
CHEMBL3 NICOTINE 4.0 1 8.82

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL513692 Ki = 0.026 nM 10.59
CHEMBL3608514 Ki = 0.094 nM 10.03
CHEMBL3608516 Ki = 0.12 nM 9.92
CHEMBL1076903 VARENICLINE Ki = 0.12 nM 9.92
CHEMBL243971 Ki = 0.13 nM 9.89
CHEMBL3608511 Ki = 0.18 nM 9.74
CHEMBL3608510 Ki = 0.22 nM 9.66
CHEMBL3608518 Ki = 0.27 nM 9.57
CHEMBL3608512 Ki = 0.29 nM 9.54
CHEMBL3608513 Ki = 0.38 nM 9.42
CHEMBL3608515 Ki = 0.81 nM 9.09
CHEMBL3608517 Ki = 0.9 nM 9.05
CHEMBL3 NICOTINE Ki = 1.5 nM 8.82