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Assay Detail

CHEMBL3610684

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at rat alpha4beta2 nAChR expressed in Xenopus laevis oocytes assessed as inhibition of acetylcholine-induced mean current response by two-electrode voltage clamp electrophysiology assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907596)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Synthesis, nicotinic acetylcholine receptor binding, in vitro and in vivo pharmacology properties of 3'-(substituted pyridinyl)-deschloroepibatidine analogs.
Ondachi PW, Ye Z, Castro AH, Luetje CW, Damaj MI, Mascarella SW, Navarro HA, Carroll FI.
Bioorg Med Chem (2015) 23 : 5693 -5701
PubMed 26233800 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.63 6.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1076903 VARENICLINE 4.0 1 6.70
CHEMBL3608514 1 5.92
CHEMBL243971 1 5.89
CHEMBL3608518 1 5.75
CHEMBL3608516 1 5.57
CHEMBL3608511 1 5.54
CHEMBL3608515 1 5.19
CHEMBL3608510 1 5.15
CHEMBL3608512 1 4.96

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1076903 VARENICLINE IC50 = 200.0 nM 6.70
CHEMBL3608514 IC50 = 1200.0 nM 5.92
CHEMBL243971 IC50 = 1300.0 nM 5.89
CHEMBL3608518 IC50 = 1800.0 nM 5.75
CHEMBL3608516 IC50 = 2700.0 nM 5.57
CHEMBL3608511 IC50 = 2900.0 nM 5.54
CHEMBL3608515 IC50 = 6400.0 nM 5.19
CHEMBL3608510 IC50 = 7100.0 nM 5.15
CHEMBL3608512 IC50 = 11000.0 nM 4.96