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Assay Detail

CHEMBL3615166

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human PDE3A using FAM-cAMP by fluorescence polarization assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

cGMP-inhibited 3',5'-cyclic phosphodiesterase 3A (CHEMBL241)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and discovery of 2-(4-(1H-tetrazol-5-yl)-1H-pyrazol-1-yl)-4-(4-phenyl)thiazole derivatives as cardiotonic agents via inhibition of PDE3.
Duan LM, Yu HY, Li YL, Jia CJ.
Bioorg Med Chem (2015) 23 : 6111 -6117
PubMed 26319621 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 5.30 6.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3614035 1 6.62
CHEMBL3614036 1 5.84
CHEMBL3614037 1 5.72
CHEMBL3614033 1 5.61
CHEMBL3614032 1 5.52
CHEMBL3614034 1 5.44
CHEMBL3614038 1 5.21
CHEMBL3614039 1 5.16
CHEMBL3614040 1 5.13
CHEMBL3614031 1 5.09
CHEMBL3614041 1 5.04
CHEMBL3614042 1 5.00
CHEMBL17423 VESNARINONE 2.0 1 4.95
CHEMBL3614043 1 4.87
CHEMBL3614044 1 4.83
CHEMBL3614045 1 4.79

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3614035 IC50 = 240.0 nM 6.62
CHEMBL3614036 IC50 = 1450.0 nM 5.84
CHEMBL3614037 IC50 = 1890.0 nM 5.72
CHEMBL3614033 IC50 = 2450.0 nM 5.61
CHEMBL3614032 IC50 = 2990.0 nM 5.52
CHEMBL3614034 IC50 = 3620.0 nM 5.44
CHEMBL3614038 IC50 = 6230.0 nM 5.21
CHEMBL3614039 IC50 = 6890.0 nM 5.16
CHEMBL3614040 IC50 = 7430.0 nM 5.13
CHEMBL3614031 IC50 = 8150.0 nM 5.09
CHEMBL3614041 IC50 = 9120.0 nM 5.04
CHEMBL3614042 IC50 = 9890.0 nM 5.00
CHEMBL17423 VESNARINONE IC50 = 11210.0 nM 4.95
CHEMBL3614043 IC50 = 13440.0 nM 4.87
CHEMBL3614044 IC50 = 14630.0 nM 4.83
CHEMBL3614045 IC50 = 16420.0 nM 4.79