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Assay Detail

CHEMBL3619271

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Functional
Agonist activity at human recombinant mGlu2 receptor expressed in AV12 cells assessed as inhibition of forskolin-stimulated cAMP production after 1 hr by fluorescence assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type AV12
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Metabotropic glutamate receptor 2 (CHEMBL5137)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and Pharmacological Characterization of C4-(Thiotriazolyl)-substituted-2-aminobicyclo[3.1.0]hexane-2,6-dicarboxylates. Identification of (1R,2S,4R,5R,6R)-2-Amino-4-(1H-1,2,4-triazol-3-ylsulfanyl)bicyclo[3.1.0]hexane-2,6-dicarboxylic Acid (LY2812223), a Highly Potent, Functionally Selective mGlu2 Receptor Agonist.
Monn JA, Prieto L, Taboada L, Hao J, Reinhard MR, Henry SS, Beadle CD, Walton L, Man T, Rudyk H, Clark B, Tupper D, Baker SR, Lamas C, Montero C, Marcos A, Blanco J, Bures M, Clawson DK, Atwell S, Lu F, Wang J, Russell M, Heinz BA, Wang X, Carter JH, Getman BG, Catlow JT, Swanson S, Johnson BG, Shaw DB, McKinzie DL.
J Med Chem (2015) 58 : 7526 -7548
PubMed 26313429 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 7.97 9.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3616857 1 9.24
CHEMBL3616856 1 9.19
CHEMBL3616851 1 8.35
CHEMBL3616860 1 8.28
CHEMBL3616847 1 8.25
CHEMBL3616858 1 8.19
CHEMBL8759 EGLUMETAD 2.0 1 8.15
CHEMBL3616848 1 7.98
CHEMBL3616854 1 7.95
CHEMBL3616850 1 7.93
CHEMBL3616853 1 7.91
CHEMBL3616849 1 7.86
CHEMBL3616852 1 7.78
CHEMBL3616855 1 7.35
CHEMBL3616859 1 7.13
CHEMBL2381651 1 5.93

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3616857 EC50 = 0.58 nM 9.24
CHEMBL3616856 EC50 = 0.65 nM 9.19
CHEMBL3616851 EC50 = 4.5 nM 8.35
CHEMBL3616860 EC50 = 5.2 nM 8.28
CHEMBL3616847 EC50 = 5.6 nM 8.25
CHEMBL3616858 EC50 = 6.4 nM 8.19
CHEMBL8759 EGLUMETAD EC50 = 7.0 nM 8.15
CHEMBL3616848 EC50 = 10.4 nM 7.98
CHEMBL3616854 EC50 = 11.2 nM 7.95
CHEMBL3616850 EC50 = 11.8 nM 7.93
CHEMBL3616853 EC50 = 12.4 nM 7.91
CHEMBL3616849 EC50 = 13.9 nM 7.86
CHEMBL3616852 EC50 = 16.5 nM 7.78
CHEMBL3616855 EC50 = 44.3 nM 7.35
CHEMBL3616859 EC50 = 74.0 nM 7.13
CHEMBL2381651 EC50 = 1170.0 nM 5.93