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Assay Detail

CHEMBL3620140

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at recombinant mouse alpha4beta2 nAChR expressed in HEK293 cells assessed as inhibition of acetylcholine-induced effect after 30 min by FLIPR potential blue assay
2
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Autocuration

Publication

Exploration of the molecular architecture of the orthosteric binding site in the α4β2 nicotinic acetylcholine receptor with analogs of 3-(dimethylamino)butyl dimethylcarbamate (DMABC) and 1-(pyridin-3-yl)-1,4-diazepane.
Bach TB, Jensen AA, Petersen JG, Sørensen TE, Della Volpe S, Liu J, Blaazer AR, van Muijlwijk-Koezen JE, Balle T, Frølund B.
Eur J Med Chem (2015) 102 : 425 -444
PubMed 26301559 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 5.96 5.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3616574 2 5.96

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3616574 IC50 = 1100.0 nM 5.96
CHEMBL3616574 IC50 = 1096.48 nM 5.96