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Assay Detail

CHEMBL3620669

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of sEH (unknown origin) using PHOME as substrate assessed as formation of 6-methoxy-2-naphthaldehyde measured during 1 hr by fluorescence photometric analysis
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bifunctional epoxide hydrolase 2 (CHEMBL2409)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Soluble epoxide hydrolase inhibitory activity of anthraquinone components from Aloe.
Sun YN, Kim JH, Li W, Jo AR, Yan XT, Yang SY, Kim YH.
Bioorg Med Chem (2015) 23 : 6659 -6665
PubMed 26372074 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.11 7.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL215387 1 7.96
CHEMBL3617999 1 5.39
CHEMBL3617998 1 4.90
CHEMBL3617997 1 4.86
CHEMBL3617995 1 4.81
CHEMBL3617996 1 4.70
CHEMBL40275 ALOE-EMODIN 1 4.57
CHEMBL497001 ALOIN -1.0 1 4.43
CHEMBL3617994 1 4.39

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL215387 IC50 = 10.9 nM 7.96
CHEMBL3617999 IC50 = 4100.0 nM 5.39
CHEMBL3617998 IC50 = 12500.0 nM 4.90
CHEMBL3617997 IC50 = 13800.0 nM 4.86
CHEMBL3617995 IC50 = 15600.0 nM 4.81
CHEMBL3617996 IC50 = 20200.0 nM 4.70
CHEMBL40275 ALOE-EMODIN IC50 = 26800.0 nM 4.57
CHEMBL497001 ALOIN IC50 = 37400.0 nM 4.43
CHEMBL3617994 IC50 = 41100.0 nM 4.39