Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3625146

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human orexin 2 receptor expressed in CHO cells assessed as inhibition of orexin-A-induced intracellular calcium level after 60 mins by FLIPR assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Orexin receptor type 2 (CHEMBL4792)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of diazepane amide DORAs and 2-SORAs enabled by exploration of isosteric quinazoline replacements.
Roecker AJ, Mercer SP, Bergman JM, Gilbert KF, Kuduk SD, Harrell CM, Garson SL, Fox SV, Gotter AL, Tannenbaum PL, Prueksaritanont T, Cabalu TD, Cui D, Lemaire W, Winrow CJ, Renger JJ, Coleman PJ.
Bioorg Med Chem Lett (2015) 25 : 4992 -4999
PubMed 25613676 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.19 9.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1083659 SUVOREXANT 4.0 1 9.40
CHEMBL3622325 1 7.58
CHEMBL1083358 1 7.57
CHEMBL3622332 1 7.57
CHEMBL3622326 1 7.52
CHEMBL3622327 1 7.42
CHEMBL3622331 1 7.37
CHEMBL3622333 1 7.36
CHEMBL3622330 1 7.28
CHEMBL3622329 1 7.00
CHEMBL3622328 1 6.92
CHEMBL3622337 1 6.61
CHEMBL3622336 1 5.58
CHEMBL3622323 1 5.50
CHEMBL3622335 1 -
CHEMBL3622334 1 -
CHEMBL3622322 1 -
CHEMBL3622324 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1083659 SUVOREXANT IC50 = 0.4 nM 9.40
CHEMBL3622325 IC50 = 26.0 nM 7.58
CHEMBL1083358 IC50 = 27.0 nM 7.57
CHEMBL3622332 IC50 = 27.0 nM 7.57
CHEMBL3622326 IC50 = 30.0 nM 7.52
CHEMBL3622327 IC50 = 38.0 nM 7.42
CHEMBL3622331 IC50 = 43.0 nM 7.37
CHEMBL3622333 IC50 = 44.0 nM 7.36
CHEMBL3622330 IC50 = 52.0 nM 7.28
CHEMBL3622329 IC50 = 99.0 nM 7.00
CHEMBL3622328 IC50 = 120.0 nM 6.92
CHEMBL3622337 IC50 = 245.0 nM 6.61
CHEMBL3622336 IC50 = 2600.0 nM 5.58
CHEMBL3622323 IC50 = 3200.0 nM 5.50
CHEMBL3622335 IC50 > 10000.0 nM -
CHEMBL3622334 IC50 > 10000.0 nM -
CHEMBL3622322 IC50 > 10000.0 nM -
CHEMBL3622324 IC50 > 10000.0 nM -