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Assay Detail

CHEMBL3625645

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant JAK1 (unknown origin) using 5-FAM-KKKKEEIYFFFG-OH substrate and ATP incubated for 180 mins by scintillation counting method
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK1 (CHEMBL2835)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Highly Selective JAK2 Inhibitor, BMS-911543, for the Treatment of Myeloproliferative Neoplasms.
Wan H, Schroeder GM, Hart AC, Inghrim J, Grebinski J, Tokarski JS, Lorenzi MV, You D, Mcdevitt T, Penhallow B, Vuppugalla R, Zhang Y, Gu X, Iyer R, Lombardo LJ, Trainor GL, Ruepp S, Lippy J, Blat Y, Sack JS, Khan JA, Stefanski K, Sleczka B, Mathur A, Sun JH, Wong MK, Wu DR, Li P, Gupta A, Arunachalam PN, Pragalathan B, Narayanan S, K C N, Kuppusamy P, Purandare AV.
ACS Med Chem Lett (2015) 6 : 850 -855
PubMed 26288683 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 6.96 7.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3622150 1 7.80
CHEMBL3622143 1 7.72
CHEMBL3622144 1 7.66
CHEMBL3622145 1 7.40
CHEMBL3622146 1 7.32
CHEMBL3545215 BMS-911543 2.0 1 7.12
CHEMBL3622142 1 6.60
CHEMBL3622148 1 6.48
CHEMBL3622149 1 6.27
CHEMBL3622147 1 5.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3622150 IC50 = 16.0 nM 7.80
CHEMBL3622143 IC50 = 19.0 nM 7.72
CHEMBL3622144 IC50 = 22.0 nM 7.66
CHEMBL3622145 IC50 = 40.0 nM 7.40
CHEMBL3622146 IC50 = 48.0 nM 7.32
CHEMBL3545215 BMS-911543 IC50 = 75.0 nM 7.12
CHEMBL3622142 IC50 = 250.0 nM 6.60
CHEMBL3622148 IC50 = 330.0 nM 6.48
CHEMBL3622149 IC50 = 540.0 nM 6.27
CHEMBL3622147 IC50 = 6000.0 nM 5.22