Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3635178

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of BACE1 in human SKNBE2 cells expressing wild type human APP695 assessed as reduction in amyloid beta-42 production after 18 hrs by sandwich alphalisa assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type SKNBE2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-secretase 1 (CHEMBL4822)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

1,4-Oxazine β-Secretase 1 (BACE1) Inhibitors: From Hit Generation to Orally Bioavailable Brain Penetrant Leads.
Rombouts FJ, Tresadern G, Delgado O, Martínez-Lamenca C, Van Gool M, García-Molina A, Alonso de Diego SA, Oehlrich D, Prokopcova H, Alonso JM, Austin N, Borghys H, Van Brandt S, Surkyn M, De Cleyn M, Vos A, Alexander R, Macdonald G, Moechars D, Gijsen H, Trabanco AA.
J Med Chem (2015) 58 : 8216 -8235
PubMed 26378740 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 7.53 8.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3634341 1 8.48
CHEMBL2403769 1 8.39
CHEMBL3634340 1 8.28
CHEMBL3634123 1 8.27
CHEMBL3634342 1 8.09
CHEMBL3634122 1 8.04
CHEMBL3634125 1 8.04
CHEMBL3634128 1 7.89
CHEMBL3634129 1 7.85
CHEMBL3634126 1 7.47
CHEMBL3634124 1 7.03
CHEMBL3634338 1 6.59
CHEMBL3634127 1 6.49
CHEMBL3634130 1 6.22
CHEMBL3634339 1 5.84

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3634341 IC50 = 3.3 nM 8.48
CHEMBL2403769 IC50 = 4.1 nM 8.39
CHEMBL3634340 IC50 = 5.2 nM 8.28
CHEMBL3634123 IC50 = 5.4 nM 8.27
CHEMBL3634342 IC50 = 8.1 nM 8.09
CHEMBL3634122 IC50 = 9.1 nM 8.04
CHEMBL3634125 IC50 = 9.1 nM 8.04
CHEMBL3634128 IC50 = 13.0 nM 7.89
CHEMBL3634129 IC50 = 14.0 nM 7.85
CHEMBL3634126 IC50 = 34.0 nM 7.47
CHEMBL3634124 IC50 = 93.0 nM 7.03
CHEMBL3634338 IC50 = 257.0 nM 6.59
CHEMBL3634127 IC50 = 324.0 nM 6.49
CHEMBL3634130 IC50 = 603.0 nM 6.22
CHEMBL3634339 IC50 = 1445.0 nM 5.84