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Assay Detail

CHEMBL3636899

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of B-RAF V600E mutant in human SK-MEL-28 cells assessed as phosphorylation of ERK
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type SK-MEL-28
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase B-raf (CHEMBL5145)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of RAF265: A Potent mut-B-RAF Inhibitor for the Treatment of Metastatic Melanoma.
Williams TE, Subramanian S, Verhagen J, McBride CM, Costales A, Sung L, Antonios-McCrea W, McKenna M, Louie AK, Ramurthy S, Levine B, Shafer CM, Machajewski T, Renhowe PA, Appleton BA, Amiri P, Chou J, Stuart D, Aardalen K, Poon D.
ACS Med Chem Lett (2015) 6 : 961 -965
PubMed 26396681 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 6.70 7.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3632721 1 7.38
CHEMBL3335371 1 7.36
CHEMBL3632719 1 6.92
CHEMBL3632720 1 6.89
CHEMBL558752 RAF-265 2.0 1 6.85
CHEMBL3632723 1 6.68
CHEMBL470808 1 6.55
CHEMBL3632717 1 6.41
CHEMBL3632718 1 6.32
CHEMBL3632724 1 6.21
CHEMBL3632722 1 6.11

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3632721 IC50 = 42.0 nM 7.38
CHEMBL3335371 IC50 = 44.0 nM 7.36
CHEMBL3632719 IC50 = 120.0 nM 6.92
CHEMBL3632720 IC50 = 130.0 nM 6.89
CHEMBL558752 RAF-265 IC50 = 140.0 nM 6.85
CHEMBL3632723 IC50 = 210.0 nM 6.68
CHEMBL470808 IC50 = 280.0 nM 6.55
CHEMBL3632717 IC50 = 390.0 nM 6.41
CHEMBL3632718 IC50 = 480.0 nM 6.32
CHEMBL3632724 IC50 = 620.0 nM 6.21
CHEMBL3632722 IC50 = 780.0 nM 6.11