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Assay Detail

CHEMBL3705102

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding Assay: The secondary assay was a time resolved-FRET LanthaScreen Kinase Binding Assay. (Invitrogen Corporation, Carlsbad, Calif.). This assay evaluates the ability of the test compound to compete with a fluorescently-labeled tracer molecule to bind in the ATP pocket of a kinase.
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

3-phosphoinositide-dependent protein kinase 1 (CHEMBL2534)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Substituted 3-(1H-benzo[d]imidazol-2-yl)-1H-indazole analogs as inhibitors of the PDK1 kinase
(2013)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 5.84 7.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3659990 1 7.10
CHEMBL3659991 1 7.03
CHEMBL3659971 1 6.64
CHEMBL3659972 1 6.58
CHEMBL3659976 1 6.55
CHEMBL3659978 1 6.42
CHEMBL3659974 1 6.39
CHEMBL3659980 1 6.04
CHEMBL3659975 1 5.91
CHEMBL3659977 1 5.91
CHEMBL3659984 1 5.29
CHEMBL3659981 1 5.06
CHEMBL3659986 1 4.91
CHEMBL3659982 1 4.82
CHEMBL3659983 1 4.52
CHEMBL3659987 1 4.27
CHEMBL3659985 1 -
CHEMBL3659988 1 -
CHEMBL3659989 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3659990 IC50 = 80.0 nM 7.10
CHEMBL3659991 IC50 = 94.0 nM 7.03
CHEMBL3659971 IC50 = 229.0 nM 6.64
CHEMBL3659972 IC50 = 265.0 nM 6.58
CHEMBL3659976 IC50 = 280.0 nM 6.55
CHEMBL3659978 IC50 = 385.0 nM 6.42
CHEMBL3659974 IC50 = 410.0 nM 6.39
CHEMBL3659980 IC50 = 920.0 nM 6.04
CHEMBL3659975 IC50 = 1230.0 nM 5.91
CHEMBL3659977 IC50 = 1220.0 nM 5.91
CHEMBL3659984 IC50 = 5170.0 nM 5.29
CHEMBL3659981 IC50 = 8790.0 nM 5.06
CHEMBL3659986 IC50 = 12400.0 nM 4.91
CHEMBL3659982 IC50 = 15300.0 nM 4.82
CHEMBL3659983 IC50 = 30300.0 nM 4.52
CHEMBL3659987 IC50 = 53900.0 nM 4.27
CHEMBL3659985 IC50 > 100000.0 nM -
CHEMBL3659988 IC50 > 100000.0 nM -
CHEMBL3659989 IC50 > 100000.0 nM -