Assay Detail
Binding
CHEMBL3705207
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Enzymatic Assay: Compounds were tested in an enzymatic assay using human ERK-2 (Mitogen Activated Kinase 1), recombinantly expressed as an n-terminal 6-His fusion protein in E. coli and corresponding to aa 8-360. The substrate used was the fluorescent Omnia peptide S/T17 (Invitrogen of Carlsbad, Calif.; Cat. KNZ1171C). Test compounds were diluted in dimethylsulfoxide (DMSO) in 3-fold serial dilutions at 100× final concentrations. In addition to compound, the assay contained 50 mM HEPES [pH 7.3], 10 mM MgCl2, 1 mM DTT, 0.005% Triton-X100, 5 nM ERK-2 enzyme, 6.25 μM S/T17 peptide substrate and 25 μM ATP (corresponding to the observed Km) for a total reaction volume of 25 μL. The assay was run at ambient temperature in a white 384-well polypropylene plate (Nunc, Inc of Naperville, Ill.; Cat. 267462) collecting data every 50 seconds for approximately 30 minutes on an Envision plate reader (PerkinElmer, Inc. of Waltham, Mass.); Excitation 340 nm/Emission 495 nm.
42
Total Activities
42
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Serine/threonine kinase inhibitors
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 42 | 8.12 | 9.10 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3675723 | — | — | 1 | 9.10 |
| CHEMBL3680608 | — | — | 1 | 8.80 |
| CHEMBL3680610 | — | — | 1 | 8.66 |
| CHEMBL3680609 | — | — | 1 | 8.62 |
| CHEMBL3680618 | — | — | 1 | 8.59 |
| CHEMBL3675720 | — | — | 1 | 8.54 |
| CHEMBL3675718 | — | — | 1 | 8.54 |
| CHEMBL3675706 | — | — | 1 | 8.52 |
| CHEMBL3409576 | — | — | 1 | 8.52 |
| CHEMBL3675719 | — | — | 1 | 8.51 |
| CHEMBL3675701 | — | — | 1 | 8.49 |
| CHEMBL3675711 | — | — | 1 | 8.48 |
| CHEMBL3544964 | RAVOXERTINIB | 2.0 | 1 | 8.48 |
| CHEMBL3675703 | — | — | 1 | 8.42 |
| CHEMBL3409585 | — | — | 1 | 8.42 |
| CHEMBL3409588 | — | — | 1 | 8.41 |
| CHEMBL3675722 | — | — | 1 | 8.41 |
| CHEMBL3675702 | — | — | 1 | 8.40 |
| CHEMBL3680616 | — | — | 1 | 8.40 |
| CHEMBL3675715 | — | — | 1 | 8.26 |
| CHEMBL3675721 | — | — | 1 | 8.24 |
| CHEMBL3409609 | — | — | 1 | 8.20 |
| CHEMBL3675707 | — | — | 1 | 8.20 |
| CHEMBL3680611 | — | — | 1 | 8.07 |
| CHEMBL3675717 | — | — | 1 | 8.06 |
| CHEMBL3680614 | — | — | 1 | 8.01 |
| CHEMBL3675713 | — | — | 1 | 7.98 |
| CHEMBL3675708 | — | — | 1 | 7.94 |
| CHEMBL3680613 | — | — | 1 | 7.92 |
| CHEMBL3675704 | — | — | 1 | 7.87 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3675723 | — | IC50 | = | 0.8 | nM | 9.10 |
| CHEMBL3680608 | — | IC50 | = | 1.6 | nM | 8.80 |
| CHEMBL3680610 | — | IC50 | = | 2.2 | nM | 8.66 |
| CHEMBL3680609 | — | IC50 | = | 2.4 | nM | 8.62 |
| CHEMBL3680618 | — | IC50 | = | 2.6 | nM | 8.59 |
| CHEMBL3675720 | — | IC50 | = | 2.9 | nM | 8.54 |
| CHEMBL3675718 | — | IC50 | = | 2.9 | nM | 8.54 |
| CHEMBL3675706 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3409576 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3675719 | — | IC50 | = | 3.1 | nM | 8.51 |
| CHEMBL3675701 | — | IC50 | = | 3.2 | nM | 8.49 |
| CHEMBL3544964 | RAVOXERTINIB | IC50 | = | 3.3 | nM | 8.48 |
| CHEMBL3675711 | — | IC50 | = | 3.3 | nM | 8.48 |
| CHEMBL3675703 | — | IC50 | = | 3.8 | nM | 8.42 |
| CHEMBL3409585 | — | IC50 | = | 3.8 | nM | 8.42 |
| CHEMBL3409588 | — | IC50 | = | 3.9 | nM | 8.41 |
| CHEMBL3675722 | — | IC50 | = | 3.9 | nM | 8.41 |
| CHEMBL3675702 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3680616 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3675715 | — | IC50 | = | 5.5 | nM | 8.26 |
| CHEMBL3675721 | — | IC50 | = | 5.7 | nM | 8.24 |
| CHEMBL3409609 | — | IC50 | = | 6.3 | nM | 8.20 |
| CHEMBL3675707 | — | IC50 | = | 6.3 | nM | 8.20 |
| CHEMBL3680611 | — | IC50 | = | 8.5 | nM | 8.07 |
| CHEMBL3675717 | — | IC50 | = | 8.7 | nM | 8.06 |
| CHEMBL3680614 | — | IC50 | = | 9.8 | nM | 8.01 |
| CHEMBL3675713 | — | IC50 | = | 10.4 | nM | 7.98 |
| CHEMBL3675708 | — | IC50 | = | 11.5 | nM | 7.94 |
| CHEMBL3680613 | — | IC50 | = | 12.1 | nM | 7.92 |
| CHEMBL3675704 | — | IC50 | = | 13.6 | nM | 7.87 |
| CHEMBL3675716 | — | IC50 | = | 13.7 | nM | 7.86 |
| CHEMBL3675709 | — | IC50 | = | 13.9 | nM | 7.86 |
| CHEMBL3680615 | — | IC50 | = | 14.5 | nM | 7.84 |
| CHEMBL3409594 | — | IC50 | = | 15.6 | nM | 7.81 |
| CHEMBL3675714 | — | IC50 | = | 15.9 | nM | 7.80 |
| CHEMBL3680612 | — | IC50 | = | 15.8 | nM | 7.80 |
| CHEMBL3409586 | — | IC50 | = | 35.7 | nM | 7.45 |
| CHEMBL3675712 | — | IC50 | = | 39.5 | nM | 7.40 |
| CHEMBL3409598 | — | IC50 | = | 51.8 | nM | 7.29 |
| CHEMBL3675710 | — | IC50 | = | 53.0 | nM | 7.28 |
| CHEMBL3675705 | — | IC50 | = | 145.6 | nM | 6.84 |
| CHEMBL3680617 | — | IC50 | = | 249.1 | nM | 6.60 |