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Assay Detail

CHEMBL3706028

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Binding
Inhibition Assay: In vitro Shp2 PTP activity inhibition assay for determination of IC50 was performed with a recombinant GST-Shp2 PTP domain protein using 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP, Invitrogen) as the substrate similar to that described previously (Chen, et al., Discovery of a novel shp2 protein tyrosine phosphatase inhibitor. Mol Pharmacol 2006; 70:562-70).
ChEMBL ↗
70
Total Activities
67
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein phosphatase non-receptor type 11 (CHEMBL3864) ↗
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Carboxy isatin hydrazones and their esters as Shp2 inhibitors
(2014)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 70 5.11 6.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3693872 — — 1 6.16
CHEMBL3693874 — — 3 5.96
CHEMBL3693873 — — 1 5.82
CHEMBL3693875 — — 1 5.60
CHEMBL3693882 — — 1 5.55
CHEMBL3693876 — — 1 5.55
CHEMBL3693922 — — 1 5.52
CHEMBL3693878 — — 1 5.50
CHEMBL3693894 — — 1 5.47
CHEMBL3693879 — — 1 5.39
CHEMBL3693904 — — 1 5.36
CHEMBL3693895 — — 1 5.35
CHEMBL3693914 — — 1 5.35
CHEMBL3693901 — — 1 5.34
CHEMBL3693887 — — 1 5.34
CHEMBL3693916 — — 1 5.33
CHEMBL3693912 — — 1 5.32
CHEMBL3693909 — — 1 5.32
CHEMBL3693907 — — 1 5.29
CHEMBL3693927 — — 1 5.27
CHEMBL3693886 — — 1 5.24
CHEMBL3693881 — — 1 5.24
CHEMBL3693900 — — 1 5.22
CHEMBL3693880 — — 2 5.21
CHEMBL3693883 — — 1 5.20
CHEMBL3693897 — — 1 5.19
CHEMBL3693919 — — 1 5.16
CHEMBL3693885 — — 1 5.16
CHEMBL3693905 — — 1 5.15
CHEMBL3693893 — — 1 5.12

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3693872 — IC50 = 700.0 nM 6.16
CHEMBL3693874 — IC50 = 1100.0 nM 5.96
CHEMBL3693874 — IC50 = 1300.0 nM 5.89
CHEMBL3693873 — IC50 = 1500.0 nM 5.82
CHEMBL3693875 — IC50 = 2500.0 nM 5.60
CHEMBL3693876 — IC50 = 2800.0 nM 5.55
CHEMBL3693882 — IC50 = 2800.0 nM 5.55
CHEMBL3693922 — IC50 = 3000.0 nM 5.52
CHEMBL3693878 — IC50 = 3200.0 nM 5.50
CHEMBL3693894 — IC50 = 3400.0 nM 5.47
CHEMBL3693879 — IC50 = 4100.0 nM 5.39
CHEMBL3693904 — IC50 = 4400.0 nM 5.36
CHEMBL3693895 — IC50 = 4500.0 nM 5.35
CHEMBL3693914 — IC50 = 4500.0 nM 5.35
CHEMBL3693901 — IC50 = 4600.0 nM 5.34
CHEMBL3693887 — IC50 = 4600.0 nM 5.34
CHEMBL3693916 — IC50 = 4700.0 nM 5.33
CHEMBL3693912 — IC50 = 4800.0 nM 5.32
CHEMBL3693909 — IC50 = 4800.0 nM 5.32
CHEMBL3693907 — IC50 = 5100.0 nM 5.29
CHEMBL3693927 — IC50 = 5400.0 nM 5.27
CHEMBL3693886 — IC50 = 5700.0 nM 5.24
CHEMBL3693881 — IC50 = 5700.0 nM 5.24
CHEMBL3693900 — IC50 = 6000.0 nM 5.22
CHEMBL3693880 — IC50 = 6100.0 nM 5.21
CHEMBL3693883 — IC50 = 6300.0 nM 5.20
CHEMBL3693897 — IC50 = 6400.0 nM 5.19
CHEMBL3693919 — IC50 = 6900.0 nM 5.16
CHEMBL3693885 — IC50 = 6900.0 nM 5.16
CHEMBL3693905 — IC50 = 7100.0 nM 5.15
CHEMBL3693893 — IC50 = 7600.0 nM 5.12
CHEMBL3693913 — IC50 = 7700.0 nM 5.11
CHEMBL3693911 — IC50 = 7800.0 nM 5.11
CHEMBL3693892 — IC50 = 8000.0 nM 5.10
CHEMBL3693874 — IC50 = 8200.0 nM 5.09
CHEMBL3693906 — IC50 = 8100.0 nM 5.09
CHEMBL3693902 — IC50 = 8100.0 nM 5.09
CHEMBL3693891 — IC50 = 8200.0 nM 5.09
CHEMBL3693924 — IC50 = 8300.0 nM 5.08
CHEMBL3693877 — IC50 = 8300.0 nM 5.08
CHEMBL3693903 — IC50 = 8400.0 nM 5.08
CHEMBL3693898 — IC50 = 8500.0 nM 5.07
CHEMBL3693926 — IC50 = 9000.0 nM 5.05
CHEMBL3693908 — IC50 = 9000.0 nM 5.05
CHEMBL3693899 — IC50 = 9100.0 nM 5.04
CHEMBL3693918 — IC50 = 9300.0 nM 5.03
CHEMBL3693930 — IC50 = 9600.0 nM 5.02
CHEMBL3693890 — IC50 = 9900.0 nM 5.00
CHEMBL3693910 — IC50 = 10400.0 nM 4.98
CHEMBL3693921 — IC50 = 11300.0 nM 4.95