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Assay Detail

CHEMBL3707743

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Electrophysiological Assay: Patch voltage clamp electrophysiology allows for the direct measurement and quantification of block of voltage-gated sodium channels (NaV's), and allows the determination of the time- and voltage-dependence of block which has been interpreted as differential binding to the resting, open, and inactivated states of the sodium channel (Hille, B., Journal of General Physiology (1977), 69: 497-515).The following patch voltage clamp electrophysiology studies were performed on representative compounds of the invention using human embryonic kidney cells (HEK), permanently transfected with an expression vector containing the full-length cDNA coding for the desired human sodium channel alpha -subunit, grown in culture media containing 10% FBS, 1% PSG, and 0.5 mg/mL G418 at 37 C. with 5% CO2. HEK cells used for the electrophysiology (EP) recordings had a passage number of less than 40 for all studies and were used within three days from the time of plating.
228
Total Activities
210
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type HEK293
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Sodium channel protein type 9 subunit alpha (CHEMBL4296)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

N-substituted benzamides and methods of use thereof
(2015)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 228 7.38 9.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3657851 1 9.52
CHEMBL3657855 1 9.40
CHEMBL3662236 1 9.00
CHEMBL3662238 1 9.00
CHEMBL3657877 1 8.88
CHEMBL3657932 1 8.85
CHEMBL3662094 3 8.70
CHEMBL3662081 1 8.70
CHEMBL3662177 1 8.70
CHEMBL3662192 1 8.70
CHEMBL3662093 1 8.70
CHEMBL3657840 1 8.66
CHEMBL3662122 2 8.52
CHEMBL3662193 1 8.52
CHEMBL3657854 1 8.52
CHEMBL3658002 1 8.52
CHEMBL3662205 1 8.52
CHEMBL3662229 1 8.51
CHEMBL3657846 1 8.51
CHEMBL3657837 1 8.48
CHEMBL3657929 2 8.44
CHEMBL3654182 1 8.42
CHEMBL3662190 1 8.40
CHEMBL3662078 1 8.40
CHEMBL3657967 1 8.40
CHEMBL3657950 1 8.40
CHEMBL3657934 1 8.37
CHEMBL3657839 1 8.34
CHEMBL3658000 1 8.30
CHEMBL3658005 1 8.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3657851 IC50 = 0.3 nM 9.52
CHEMBL3657855 IC50 = 0.4 nM 9.40
CHEMBL3662238 IC50 = 1.0 nM 9.00
CHEMBL3662236 IC50 = 1.0 nM 9.00
CHEMBL3657877 IC50 = 1.31 nM 8.88
CHEMBL3657932 IC50 = 1.4 nM 8.85
CHEMBL3662192 IC50 = 2.0 nM 8.70
CHEMBL3662093 IC50 = 2.0 nM 8.70
CHEMBL3662177 IC50 = 2.0 nM 8.70
CHEMBL3662094 IC50 = 2.0 nM 8.70
CHEMBL3662081 IC50 = 2.0 nM 8.70
CHEMBL3662094 IC50 = 2.0 nM 8.70
CHEMBL3657840 IC50 = 2.2 nM 8.66
CHEMBL3662205 IC50 = 3.0 nM 8.52
CHEMBL3658002 IC50 = 3.0 nM 8.52
CHEMBL3657854 IC50 = 3.0 nM 8.52
CHEMBL3662193 IC50 = 3.0 nM 8.52
CHEMBL3662122 IC50 = 3.0 nM 8.52
CHEMBL3657846 IC50 = 3.1 nM 8.51
CHEMBL3662229 IC50 = 3.1 nM 8.51
CHEMBL3657837 IC50 = 3.3 nM 8.48
CHEMBL3657929 IC50 = 3.6 nM 8.44
CHEMBL3657929 IC50 = 3.6 nM 8.44
CHEMBL3654182 IC50 = 3.8 nM 8.42
CHEMBL3657967 IC50 = 4.0 nM 8.40
CHEMBL3657950 IC50 = 4.0 nM 8.40
CHEMBL3662190 IC50 = 4.0 nM 8.40
CHEMBL3662078 IC50 = 4.0 nM 8.40
CHEMBL3657934 IC50 = 4.3 nM 8.37
CHEMBL3657839 IC50 = 4.6 nM 8.34
CHEMBL3658005 IC50 = 5.0 nM 8.30
CHEMBL3658000 IC50 = 5.0 nM 8.30
CHEMBL3657805 IC50 = 5.2 nM 8.28
CHEMBL3341985 IC50 = 5.5 nM 8.26
CHEMBL3657954 IC50 = 6.0 nM 8.22
CHEMBL3662099 IC50 = 6.0 nM 8.22
CHEMBL3657954 IC50 = 6.0 nM 8.22
CHEMBL3657931 IC50 = 6.7 nM 8.17
CHEMBL3657808 IC50 = 6.9 nM 8.16
CHEMBL4115551 IC50 = 7.0 nM 8.15
CHEMBL3657887 IC50 = 7.3 nM 8.14
CHEMBL3657852 IC50 = 7.6 nM 8.12
CHEMBL3657828 IC50 = 7.7 nM 8.11
CHEMBL3662230 IC50 = 7.8 nM 8.11
CHEMBL3658020 IC50 = 8.0 nM 8.10
CHEMBL3662072 IC50 = 8.0 nM 8.10
CHEMBL3657998 IC50 = 8.0 nM 8.10
CHEMBL3662090 IC50 = 8.0 nM 8.10
CHEMBL3657806 IC50 = 8.2 nM 8.09
CHEMBL3657807 IC50 = 8.7 nM 8.06