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Assay Detail

CHEMBL3707973

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory Assay: FCPIA Characterization of RGS Inhibitory Activity: CCG-50014 (FIG. 1) was originally identified as a potential inhibitor of RGS8 and RGS16 in a polyplex high throughput screen to identify inhibitors of the RGS-Gα interaction [4]. This activity was confirmed by analyzing the effect of CCG-50014 on several different RGS proteins with freshly reordered compound using multiplexed FCPIA. CCG-50014 fully inhibited several different RGS proteins including RGS4, 8, 16, and 19, but did not have activity on RGS7 or a mutated form of RGS4 that lacks cysteine residues.
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Regulator of G-protein signaling 19 (CHEMBL3707468)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Small molecule inhibitors of RGS proteins
(2014)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 6.92 6.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1917204 1 6.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1917204 IC50 = 120.0 nM 6.92