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Assay Detail

CHEMBL3721001

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity against rat P2X3 receptor expressed in CHOK1 cells assessed as inhibition of alpha,beta-meATP-induced calcium response incubated for 20 mins by Fluo-3AM dye based FLIPR assay
22
Total Activities
22
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

P2X purinoceptor 3 (CHEMBL4824)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Diaminopyrimidines as P2X3 and P2X2/3 modulators
(2008)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 21 7.91 8.66
IC50 1 8.24 8.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3715500 1 8.66
CHEMBL494161 1 8.53
CHEMBL3717153 1 8.24
CHEMBL523173 1 8.24
CHEMBL495204 1 8.20
CHEMBL494353 1 8.15
CHEMBL494160 1 8.12
CHEMBL3718402 1 8.08
CHEMBL3715231 1 8.02
CHEMBL526307 1 8.01
CHEMBL492300 1 8.00
CHEMBL2304566 1 7.99
CHEMBL3715486 1 7.98
CHEMBL3718862 1 7.95
CHEMBL3716526 1 7.94
CHEMBL494159 1 7.93
CHEMBL492907 1 7.84
CHEMBL3718425 1 7.77
CHEMBL3716384 1 7.44
CHEMBL3716057 GEFAPIXANT 4.0 1 7.15
CHEMBL3717944 1 7.11
CHEMBL494581 1 6.99

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3715500 Ki = 2.188 nM 8.66
CHEMBL494161 Ki = 2.951 nM 8.53
CHEMBL3717153 IC50 = 5.754 nM 8.24
CHEMBL523173 Ki = 5.754 nM 8.24
CHEMBL495204 Ki = 6.31 nM 8.20
CHEMBL494353 Ki = 7.079 nM 8.15
CHEMBL494160 Ki = 7.586 nM 8.12
CHEMBL3718402 Ki = 8.318 nM 8.08
CHEMBL3715231 Ki = 9.55 nM 8.02
CHEMBL526307 Ki = 9.772 nM 8.01
CHEMBL492300 Ki = 10.0 nM 8.00
CHEMBL2304566 Ki = 10.23 nM 7.99
CHEMBL3715486 Ki = 10.47 nM 7.98
CHEMBL3718862 Ki = 11.22 nM 7.95
CHEMBL3716526 Ki = 11.48 nM 7.94
CHEMBL494159 Ki = 11.75 nM 7.93
CHEMBL492907 Ki = 14.45 nM 7.84
CHEMBL3718425 Ki = 16.98 nM 7.77
CHEMBL3716384 Ki = 36.31 nM 7.44
CHEMBL3716057 GEFAPIXANT Ki = 70.79 nM 7.15
CHEMBL3717944 Ki = 77.62 nM 7.11
CHEMBL494581 Ki = 102.33 nM 6.99