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Assay Detail

CHEMBL3721247

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Mixed type inhibition of human PFKFB3 expressed in Escherichia coli BL21 (DE3) assessed as reduction in NADH consumption using fructose-6-phosphate incubated for 15 mins by spectrophotometry based pyruvate kinase and LDH enzyme coupled kinetic assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Family of PFKFB3 inhibitors with anti-neoplastic activities
(2013)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 1 4.60 4.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3105848 1 4.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3105848 Ki = 25100.0 nM 4.60