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Assay Detail

CHEMBL3737804

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length human fusion His6 tagged LSD1 expressed in Escherichia coli BL21(DE3) cells using H3K4me2 peptide substrate preincubated for 5 mins by peroxidase-coupled method
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysine-specific histone demethylase 1A (CHEMBL6136)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and biological activity of N-alkylated analogue of NCL1, a selective inhibitor of lysine-specific demethylase 1
Ahmed Khan MN, Tsumoto H, Itoh Y, Ota Y, Suzuki M, Ogasawara D, Nakagawa H, Mizukami T, Miyata N, Suzuki T
Medchemcomm (2015) 6 : 407 -412
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 5.88 6.42

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3736127 1 6.42
CHEMBL3735310 1 5.62
CHEMBL1797639 1 5.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3736127 IC50 = 380.0 nM 6.42
CHEMBL3735310 IC50 = 2400.0 nM 5.62
CHEMBL1797639 IC50 = 2500.0 nM 5.60