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Assay Detail

CHEMBL3743579

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at P2X7R in human whole blood assessed as inhibition of LPS-induced IL-1beta production incubated for 30 mins followed by ATP addition for 30 mins by ELISA assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Blood
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

P2X purinoceptor 7 (CHEMBL4805)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel Series of Dihydropyridinone P2X7 Receptor Antagonists.
Lopez-Tapia F, Walker KA, Brotherton-Pleiss C, Caroon J, Nitzan D, Lowrie L, Gleason S, Zhao SH, Berger J, Cockayne D, Phippard D, Suttmann R, Fitch WL, Bourdet D, Rege P, Huang X, Broadbent S, Dvorak C, Zhu J, Wagner P, Padilla F, Loe B, Jahangir A, Alker A.
J Med Chem (2015) 58 : 8413 -8426
PubMed 26460788 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 7.60 8.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3739648 1 8.22
CHEMBL3740684 1 7.89
CHEMBL3739741 1 7.77
CHEMBL3740645 1 7.77
CHEMBL3740130 1 7.70
CHEMBL3741681 1 7.68
CHEMBL3741076 1 7.57
CHEMBL3740255 1 7.48
CHEMBL3741276 1 7.33
CHEMBL3741992 1 7.22
CHEMBL3740237 1 6.97

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3739648 IC50 = 6.0 nM 8.22
CHEMBL3740684 IC50 = 13.0 nM 7.89
CHEMBL3739741 IC50 = 17.0 nM 7.77
CHEMBL3740645 IC50 = 17.0 nM 7.77
CHEMBL3740130 IC50 = 20.0 nM 7.70
CHEMBL3741681 IC50 = 21.0 nM 7.68
CHEMBL3741076 IC50 = 27.0 nM 7.57
CHEMBL3740255 IC50 = 33.0 nM 7.48
CHEMBL3741276 IC50 = 47.0 nM 7.33
CHEMBL3741992 IC50 = 60.0 nM 7.22
CHEMBL3740237 IC50 = 107.0 nM 6.97