Assay Detail
Binding
CHEMBL3744149
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of hedgehog signalling in mouse C3H10T1/2 cells assessed as reduction in Smo agonist SAG induced cell differentiation into alkaline phosphatase-positive osteoblasts using pNp substrate incubated for 72 hrs
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Cell Type | C3H 10T1/2 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Synthesis and evaluation of novel N-3-benzimidazolephenylbisamide derivatives for antiproliferative and Hedgehog pathway inhibitory activity
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 7.09 | 7.77 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL473417 | VISMODEGIB | 4.0 | 1 | 7.77 |
| CHEMBL3740447 | — | — | 1 | 7.31 |
| CHEMBL3741651 | — | — | 1 | 7.20 |
| CHEMBL2031084 | — | — | 1 | 6.06 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL473417 | VISMODEGIB | IC50 | = | 17.0 | nM | 7.77 |
| CHEMBL3740447 | — | IC50 | = | 49.0 | nM | 7.31 |
| CHEMBL3741651 | — | IC50 | = | 63.0 | nM | 7.20 |
| CHEMBL2031084 | — | IC50 | = | 868.0 | nM | 6.06 |