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Assay Detail

CHEMBL3744622

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [33P]S1P from S1P1 receptor (unknown origin) expressed in HEK cell membranes after 45 to 60 mins by scintillation counting based RLB method
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sphingosine 1-phosphate receptor 1 (CHEMBL4333)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of A-971432, An Orally Bioavailable Selective Sphingosine-1-Phosphate Receptor 5 (S1P5) Agonist for the Potential Treatment of Neurodegenerative Disorders.
Hobson AD, Harris CM, van der Kam EL, Turner SC, Abibi A, Aguirre AL, Bousquet P, Kebede T, Konopacki DB, Gintant G, Kim Y, Larson K, Maull JW, Moore NS, Shi D, Shrestha A, Tang X, Zhang P, Sarris KK.
J Med Chem (2015) 58 : 9154 -9170
PubMed 26509640 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 6.27 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3741743 1 8.70
CHEMBL3739878 1 8.70
CHEMBL3739440 1 8.30
CHEMBL3740062 1 7.75
CHEMBL3741496 1 7.32
CHEMBL3741364 1 6.92
CHEMBL1973936 1 6.66
CHEMBL3741022 1 6.66
CHEMBL3741589 1 6.44
CHEMBL3740359 1 6.18
CHEMBL1968499 1 6.08
CHEMBL1971132 1 5.97
CHEMBL3740060 1 5.93
CHEMBL3742245 1 5.75
CHEMBL3742304 1 5.52
CHEMBL3740090 1 5.46
CHEMBL3740494 1 5.41
CHEMBL3742033 1 5.38
CHEMBL1987998 1 5.37
CHEMBL3742345 1 5.36
CHEMBL3740768 1 5.28
CHEMBL3741222 1 5.21
CHEMBL3742384 1 5.14
CHEMBL3741338 1 5.01
CHEMBL3740188 1 -
CHEMBL3739977 1 -
CHEMBL3740355 1 -
CHEMBL3740219 1 -
CHEMBL3742100 1 -
CHEMBL3741414 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3741743 IC50 = 2.0 nM 8.70
CHEMBL3739878 IC50 = 2.0 nM 8.70
CHEMBL3739440 IC50 = 5.0 nM 8.30
CHEMBL3740062 IC50 = 18.0 nM 7.75
CHEMBL3741496 IC50 = 48.0 nM 7.32
CHEMBL3741364 IC50 = 120.0 nM 6.92
CHEMBL1973936 IC50 = 221.0 nM 6.66
CHEMBL3741022 IC50 = 220.0 nM 6.66
CHEMBL3741589 IC50 = 362.0 nM 6.44
CHEMBL3740359 IC50 = 656.0 nM 6.18
CHEMBL1968499 IC50 = 835.0 nM 6.08
CHEMBL1971132 IC50 = 1060.0 nM 5.97
CHEMBL3740060 IC50 = 1180.0 nM 5.93
CHEMBL3742245 IC50 = 1780.0 nM 5.75
CHEMBL3742304 IC50 = 3030.0 nM 5.52
CHEMBL3740090 IC50 = 3460.0 nM 5.46
CHEMBL3740494 IC50 = 3870.0 nM 5.41
CHEMBL3742033 IC50 = 4170.0 nM 5.38
CHEMBL1987998 IC50 = 4230.0 nM 5.37
CHEMBL3742345 IC50 = 4410.0 nM 5.36
CHEMBL3740768 IC50 = 5260.0 nM 5.28
CHEMBL3741222 IC50 = 6230.0 nM 5.21
CHEMBL3742384 IC50 = 7210.0 nM 5.14
CHEMBL3741338 IC50 = 9810.0 nM 5.01
CHEMBL3740188 IC50 > 10000.0 nM -
CHEMBL3739977 IC50 > 10000.0 nM -
CHEMBL3740355 IC50 > 30000.0 nM -
CHEMBL3740219 IC50 > 10000.0 nM -
CHEMBL3742100 IC50 > 10000.0 nM -
CHEMBL3741414 IC50 < 1.0 nM -