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Assay Detail

CHEMBL3745028

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at P2X7 receptor expressed in LPS/IFNgamma-differentiated human THP1 cells assessed as inhibition of BzATP-induced IL-1beta production after 4 hrs by ELISA
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type THP-1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

P2X purinoceptor 7 (CHEMBL4805)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-activity relationship studies of pyrimidine-2,4-dione derivatives as potent P2X7 receptor antagonists.
Park JH, Lee GE, Lee SD, Ko H, Kim YC.
Eur J Med Chem (2015) 106 : 180 -193
PubMed 26547056 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 7.55 7.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3740315 1 7.92
CHEMBL3741159 1 7.85
CHEMBL3742331 1 7.77
CHEMBL3741837 1 7.64
CHEMBL3742001 1 7.52
CHEMBL3740602 1 7.50
CHEMBL3741312 1 7.34
CHEMBL28324 1 6.89

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3740315 IC50 = 12.0 nM 7.92
CHEMBL3741159 IC50 = 14.0 nM 7.85
CHEMBL3742331 IC50 = 17.0 nM 7.77
CHEMBL3741837 IC50 = 23.0 nM 7.64
CHEMBL3742001 IC50 = 30.0 nM 7.52
CHEMBL3740602 IC50 = 32.0 nM 7.50
CHEMBL3741312 IC50 = 46.0 nM 7.34
CHEMBL28324 IC50 = 129.0 nM 6.89