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Assay Detail

CHEMBL3748443

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant hepsin using Boc-QAR-AMC as substrate preincubated for 30 mins followed by substrate addition measured for 15 to 120 mins by plate reader analysis
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine protease hepsin (CHEMBL2079849)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-based design, synthesis, and biological evaluation of Leu-Arg dipeptide analogs as novel hepsin inhibitors.
Kwon H, Kim Y, Park K, Choi SA, Son SH, Byun Y.
Bioorg Med Chem Lett (2016) 26 : 310 -314
PubMed 26711145 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 12 8.47 9.08

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3745775 1 9.08
CHEMBL3747482 1 8.98
CHEMBL3356589 1 8.84
CHEMBL3747468 1 8.54
CHEMBL3746517 1 8.54
CHEMBL404190 1 8.47
CHEMBL3747618 1 7.67
CHEMBL3747575 1 7.65
CHEMBL3747752 1 -
CHEMBL3746062 1 -
CHEMBL3746635 1 -
CHEMBL3747693 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3745775 Ki = 0.83 nM 9.08
CHEMBL3747482 Ki = 1.04 nM 8.98
CHEMBL3356589 Ki = 1.45 nM 8.84
CHEMBL3747468 Ki = 2.88 nM 8.54
CHEMBL3746517 Ki = 2.91 nM 8.54
CHEMBL404190 Ki = 3.38 nM 8.47
CHEMBL3747618 Ki = 21.5 nM 7.67
CHEMBL3747575 Ki = 22.4 nM 7.65
CHEMBL3747752 Ki > 10000.0 nM -
CHEMBL3746062 Ki > 10000.0 nM -
CHEMBL3746635 Ki > 10000.0 nM -
CHEMBL3747693 Ki > 10000.0 nM -