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Assay Detail

CHEMBL3750370

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PIM kinase in luciferase expressing human KMS11 cells assessed as inhibition of phosphorylation of S6RP after 1 hr by electrochemiluminescence assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type KMS11
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Identification of N-(4-((1R,3S,5S)-3-Amino-5-methylcyclohexyl)pyridin-3-yl)-6-(2,6-difluorophenyl)-5-fluoropicolinamide (PIM447), a Potent and Selective Proviral Insertion Site of Moloney Murine Leukemia (PIM) 1, 2, and 3 Kinase Inhibitor in Clinical Trials for Hematological Malignancies.
Burger MT, Nishiguchi G, Han W, Lan J, Simmons R, Atallah G, Ding Y, Tamez V, Zhang Y, Mathur M, Muller K, Bellamacina C, Lindvall MK, Zang R, Huh K, Feucht P, Zavorotinskaya T, Dai Y, Basham S, Chan J, Ginn E, Aycinena A, Holash J, Castillo J, Langowski JL, Wang Y, Chen MY, Lambert A, Fritsch C, Kauffmann A, Pfister E, Vanasse KG, Garcia PD.
J Med Chem (2015) 58 : 8373 -8386
PubMed 26505898 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 5 6.59 7.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3105022 1 7.52
CHEMBL3651966 LGH-447 1.0 1 6.75
CHEMBL3676300 1 6.50
CHEMBL3651993 1 6.15
CHEMBL3651946 1 6.04

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3105022 EC50 = 30.0 nM 7.52
CHEMBL3651966 LGH-447 EC50 = 180.0 nM 6.75
CHEMBL3676300 EC50 = 320.0 nM 6.50
CHEMBL3651993 EC50 = 710.0 nM 6.15
CHEMBL3651946 EC50 = 910.0 nM 6.04