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Assay Detail

CHEMBL3755460

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NAMPT in human SH-SY5Y cells assessed as NAD depletion incubated for 16 hrs
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type SH-SY5Y
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nicotinamide phosphoribosyltransferase (CHEMBL1744525)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of a novel NAMPT inhibitor by combinatorial click chemistry and chemical refinement
Theeramunkong S, Galli U, Grolla AA, Caldarelli A, Travelli C, Massarotti A, Troiani MP, Alisi MA, Orsomando G, Genazzani AA, Tron GC
Medchemcomm (2015) 6 : 1891 -1897
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 9.47 10.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL566757 DAPORINAD 2.0 1 10.52
CHEMBL3753643 1 10.29
CHEMBL3753517 1 8.96
CHEMBL3752157 1 8.12

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL566757 DAPORINAD IC50 = 0.0301 nM 10.52
CHEMBL3753643 IC50 = 0.0509 nM 10.29
CHEMBL3753517 IC50 = 1.1 nM 8.96
CHEMBL3752157 IC50 = 7.6 nM 8.12