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Assay Detail

CHEMBL3756249

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human IDO1 assessed as conversion of N-formylkynurenine to kynurenine incubated for 1 hr by fluorescence analysis
12
Total Activities
12
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Indoleamine 2,3-dioxygenase 1 (CHEMBL4685)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Important Hydrogen Bond Networks in Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitor Design Revealed by Crystal Structures of Imidazoleisoindole Derivatives with IDO1.
Peng YH, Ueng SH, Tseng CT, Hung MS, Song JS, Wu JS, Liao FY, Fan YS, Wu MH, Hsiao WC, Hsueh CC, Lin SY, Cheng CY, Tu CH, Lee LC, Cheng MF, Shia KS, Shih C, Wu SY.
J Med Chem (2016) 59 : 282 -293
PubMed 26642377 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 6.38 7.72
Inhibition 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3753837 1 7.72
CHEMBL3629569 1 7.42
CHEMBL3754460 1 6.55
CHEMBL3629570 1 6.33
CHEMBL3753777 1 5.94
CHEMBL3752209 1 4.32
CHEMBL3752239 1 -
CHEMBL3747548 1 -
CHEMBL3754016 1 -
CHEMBL3752732 1 -
CHEMBL3752711 1 -
CHEMBL3752060 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3753837 IC50 = 19.0 nM 7.72
CHEMBL3629569 IC50 = 38.0 nM 7.42
CHEMBL3754460 IC50 = 279.0 nM 6.55
CHEMBL3629570 IC50 = 468.0 nM 6.33
CHEMBL3753777 IC50 = 1139.0 nM 5.94
CHEMBL3752209 IC50 = 48000.0 nM 4.32
CHEMBL3752239 IC50 > 10000.0 nM -
CHEMBL3747548 IC50 > 10000.0 nM -
CHEMBL3754016 IC50 > 10000.0 nM -
CHEMBL3752732 IC50 > 10000.0 nM -
CHEMBL3752711 Inhibition - % -
CHEMBL3752060 Inhibition - % -